Abstract
The development of recombinant DNA techniques, monoclonal antibody techniques and microchemical instrumentation has revolutionized modern biology. These biotechnologies operate in a synergistic fashion to expand enormously the repertoire of biological problems that can be effectively approached. Moreover, they have had a profound impact on shortening the time between fundamental observations and their application to human physiology and medicine. In this paper we describe a selection of ongoing projects in our laboratory to develop additional or improved components for the microchemical facility.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Hunkapiller, M., Kent, S., Caruthers, M., Dreyer, W., Jirca, J., Giffin, C., Horvath, S., Hunkapiller, T., Tempst, P., and Hood, L. (1984) Nature 310, 105–111.
Edman, P., and Begg, G. (1967) Eur. J. Biochem 1, 80–91.
O’Farrell, P. H. (1975) J. Biol. Chem 250, 4007.
Kent, S., Hood, L Aebersold, R., Teplow, D., Smith, L., Farnsworth, V., Cartier, P., Hines, W., Hughes, P., and Dodd, C. (1986) in Modern Methods in Protein Chemistry,J. J. L’Italien, ed., Plenum Press, New York, in press.
L’Italien, J. J., and Kent, S. B. H. (1984) J. Chromatography 283, 149–156
Aebersold, R. H., Teplow, D. B., Hood, L. E., and Kent, S. B. H. (1986) J. Biol. Chem 261, 4229–4238.
Aebersold, R. A., Leavitt, J., Hood, L. E., and Kent, S. B. H. This Volume.
Bravo, R. (1984) in Two-Dimensional Gel Electrophoresis of Proteins: Methods and Applications (Cells, J. E. and Bravo, R., eds ), Academic Press, Inc.
Kent, S. and Clark-Lewis, I. (1985) in Synthetic Peptides in Biology and Medicine (Alitalo, K., Partanen, P. and Vaheri, A., eds), pp. 29–57, Elsevier Science Publishers B.V.
Woo, D. D., Clark-Lewis, I., and Kent, S. B. H. Manuscript in Preparation.
Clark-Lewis, I., Aebersold, R., Ziltener, H., Schrader, J. W., Hood, L. E., and Kent, S. B. H. (1986) Science 231 134139.
Horvath, S. J., Firca, J. R., Hunkapiller, T., Hunkapiller, M. W., and Hood, L. Meth. Enzymol,in press.
Normanly, J., Odgen, R., Horvath, S., and Abelson, J. (1986) Nature 321, 213–219.
Schultz, S. C., and Richards, J. H. (1986) Proc. Nati. Acad. Sci. USA 83, 1588–1592.
Strauss, E. C., Kobori, J. A., Siu, G., and Hood, L. (1986) Anal. Biochem 154, 353–360.
Smith, L. M., Fung, S., Hunkapiller, M. W., Hunkapiller, T. J., and Hood, L. E. (1986) Nuc1. Acids. Res 13, 2399–2412.
Maxam, A. M., Gilbert, and Gilbert, W. (1980) Meth. Enzmol 65, 560–580.
Smith, A. J. H. (1980) Meth. Enzymol 65, 560–580.
Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connell, C., Heiner, C., Kent, S. B. H. and Hood, L. E. (1986) Nature 321, 674–679.
Caruthers, M. H. (1984) in Contemporary Topics in Polymer Science, E. J. Vandenberg, ed, Plenum Press, New York, pp. 55–71.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hood, L. et al. (1987). The Development of a Facility to Analyze and Synthesize Genes and Proteins. In: Walsh, K.A. (eds) Methods in Protein Sequence Analysis · 1986. Experimental Biology and Medicine, vol 14. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59259-480-1_2
Download citation
DOI: https://doi.org/10.1007/978-1-59259-480-1_2
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4757-5826-9
Online ISBN: 978-1-59259-480-1
eBook Packages: Springer Book Archive