Abstract
Transformation of primary tissue culture cells or cells of established lines is efficiently induced by microinjection of intact SV40 DNA or subgenomic DNA fragments which contain only the early genome region (1,2). This SV40 part codes for two related proteins, the large T-(tumor) antigen and the small t-antigen. Transformation experiments with temperature sensitive (tsA) — and small t-antigen negative mutants have shown that synthesis of T-antigen is required and sufficient for induction and maintenance of the transformed state (for review see Tooze, 3). In the meantime many details about the T-antigen have become known, but it is still unclear which of the T-antigen specific function(s) is crucial for the SV40 oncogenicity.
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© 1985 Plenum Press, New York
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Graessmann, A., Graessmann, M. (1985). The Transformation Capacity of Early SV40 DNA Fragments. In: Celis, J., Graessmann, A. (eds) Cell Transformation. NATO ASI Series, vol 91. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5009-5_6
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DOI: https://doi.org/10.1007/978-1-4684-5009-5_6
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