Abstract
Bisretinoid fluorophores of retinal pigment epithelial (RPE) lipofuscin have been shown to undergo degradation in two ways, the first involving photofragmentation following photooxidation of their polyene structure and the second being enzyme-mediated and limited, thus far, to in vitro models employing horseradish peroxidase (HRP). Here we show that both of these processes impact the ubiquitin–proteasome system (UPS) of the RPE cell. By measuring the consumption of A2E and all-trans-retinal dimer by HPLC, we confirmed that both HRP-mediated and photodegradation of the compounds occurred and that in both cases the chymotrypsin-like and trypsin-like activities of the proteasome system were decreased. With HRP-mediated degradation of A2E, there was a small negative impact on cell viability that was not mitigated by elevating gluthathione in the cell.
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References
Dudek EJ, Shang F, Valverde P, Liu Q, Hobbs M, Taylor A (2005) Selectivity of the ubiquitin pathway for oxidatively modified proteins: relevance to protein precipitation diseases. FASEB J 19(12):1707–1709
Fernandes AF, Zhou J, Zhang X, Bian Q, Sparrow JR, Taylor A, Pereira P, Shang F (2008) Oxidative inactivation of the proteasome in retinal pigment epithelial cells. A potential link between oxidative stress and up-regulation of interleukin-8. J Biol Chem 283:20745–20753
Wu Y, Zhou J, Fishkin N, Rittmann BE, Sparrow JR (2011) Enzymatic degradation of A2E, a retinal pigment epithelial lipofuscin bisretinoid. J Am Chem Soc 133:849–857
Sparrow JR, Parish CA, Hashimoto M, Nakanishi K (1999) A2E, a lipofuscin fluorophore, in human retinal pigmented epithelial cells in culture. Invest Ophthalmol Vis Sci 40(12):2988–2995
Sparrow JR, Zhou J, Ben-Shabat S, Vollmer H, Itagaki Y, Nakanishi K (2002) Involvement of oxidative mechanisms in blue light induced damage to A2E-laden RPE. Invest Ophthalmol Vis Sci 43(4):1222–1227
Kim SR, Jang YP, Jockusch S, Fishkin NE, Turro NJ, Sparrow JR (2007) The all-trans-retinal dimer series of lipofuscin pigments in retinal pigment epithelial cells in a recessive Stargardt disease model. Proc Natl Acad Sci U S A 104:19273–19278
Fahey JW, Zhang Y, Talalay P (1997) Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci U S A 94:10367–10372
Zhou J, Gao X, Cai B, Sparrow JR (2006) Indirect antioxidant protection against photooxidative processes initiated in retinal pigment epithelial cells by a lipofuscin pigment. Rejuven Res 9(2):256–263
Sparrow JR, Gregory-Roberts E, Yamamoto K, Blonska A, Ghosh SK, Ueda K, Zhou J (2012) The bisretinoids of retinal pigment epithelium. Prog Retin Eye Res 31:121–135
Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR, Maguire AM, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effective gene delivery in mice. J Clin Invest 118:1955–1964
Kong J, Kim SR, Binley K, Pata, Doi K, Mannik J, Zernant-Rajang J, Kan O, Iqball S, Naylor S, Sparrow JR, Gouras P, Allikmets R (2008) Correction of the disease phenotype in the mouse model of Stargardt disease by lentiviral gene therapy. Gene Ther 15(19):1311–1320
Radu RA, Han Y, Bui TV, Nusinowitz S, Bok D, Lichter J, Widder K, Travis GH, Mata NL (2005) Reductions in serum vitamin A arrest accumulation of toxic retinal fluorophores: a potential therapy for treatment of lipofuscin-based retinal diseases. Invest Ophthalmol Vis Sci 46:4393–4401
Maiti P, Kong J, Kim SR, Sparrow JR, Allikmets R, Rando RR (2006) Small molecule RPE65 antagonists limit the visual cycle and prevent lipofuscin formation. Biochem 45:852–860
Maeda A, Maeda T, Golczak M, Palczewski K (2008) Retinopathy in mice induced by disrupted all-trans-retinal clearance. J Biol Chem 283:26684–26693
Yoon KD, Yamamoto K, Zhou J, Sparrow JR (2011) Photo-products of retinal pigment epithelial bisretinoids react with cellular thiols. Mol Vis 17:1839–1849
Acknowledgments
This study was supported by the Edward N. and Della L. Thome Memorial Foundation, National Institutes of Health grant P30EY019007, and a grant from Research to Prevent Blindness to the Department of Ophthalmology, Columbia University.
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Sparrow, J., Zhou, J., Ghosh, S., Liu, Z. (2014). Bisretinoid Degradation and the Ubiquitin-Proteasome System. In: Ash, J., Grimm, C., Hollyfield, J., Anderson, R., LaVail, M., Bowes Rickman, C. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 801. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3209-8_75
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DOI: https://doi.org/10.1007/978-1-4614-3209-8_75
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