Abstract
The primo node (PN) was recently identified as a part of the primo network system in rats and rabbits. In this work, we studied the effects of acetylcholine, pilocarpine, and nifedipine, an antagonist of Ca2+ channels, on the membrane potential of cells in the organ-surface PN. Bath application of acetylcholine (15 μM) hyperpolarized the PN cells by 8.4 ± 5.2 mV. A similar response was induced by pilocarpine, a muscarinic agonist. In addition, acetylcholine and pilocarpine changed the interval, duration, amplitude, frequency, and the half-width of the spikes for some cases. Nifedipine depolarized PN cells by 13.4 ± 6.4 mV. Our results show that PN cells have cholinergic muscarinic receptors and voltage-dependent Ca2+ channels, indicating that the activity of the PN (i.e., contractility) can be under a cholinergic control.
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Acknowledgments
This work was supported by Industrial Strategic technology development program (10035137-2010-01) funded by the Ministry of Knowledge Economy (MKE, Korea). K. Soh thanks the Association of Korean Oriental Medicine for support.
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Park, SH., Lee, BC., Choi, CJ., Soh, KS., Ryu, P.D. (2012). Effects of Cholinergic Drugs on Membrane Potential of Cells in Organ Surface Primo Nodes. In: Soh, KS., Kang, K., Harrison, D. (eds) The Primo Vascular System. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0601-3_35
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DOI: https://doi.org/10.1007/978-1-4614-0601-3_35
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