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Establishment of Cell Lines from the Human Middle and Inner Ear Epithelial Cells

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Human Cell Transformation

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 720))

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Abstract

The middle ear infection is the most common childhood infection. In order to elucidate the cell and molecular mechanisms involved in bacterial recognition and innate immune response, we have established a stable human middle ear cell line, which has contributed to the current knowledge concerning the molecular pathogenesis of the middle ear infection. The inner ear, a sensory organ responsible for hearing and balance, is filled with inner ear fluid, and disturbance of the fluid homeostasis results in dizziness and hearing impairment. It has been suggested that the endolymphatic sac (ES) may play a critical role in the fluid homeostasis of the inner ear. We have established a stable human ES cell line and are undertaking cell and molecular characterization of this cell line.

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Acknowledgement

We would like to express our profound thanks for the guidance and assistance of Dr. Johng Rhim in establishing human middle ear and endolymphatic sac cell lines. This work was supported in part by grants DC5025 and DC6276 from the NIH, NIDCD.

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Authors and Affiliations

  1. Division of Clinical and Translational Research, House Ear Institute, 2100 West 3rd Street, Los Angeles, CA, 90057, USA

    David J. Lim

  2. Department of Cell and Neurobiology, University of Southern California, Los Angeles, CA, USA

    David J. Lim

  3. Division of Clinical and Translational Research, House Ear Institute, Los Angeles, CA, USA

    Sung K. Moon

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  1. David J. Lim
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  2. Sung K. Moon
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Correspondence to David J. Lim .

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Editors and Affiliations

  1. Uniformed Services, Department of Surgery, University of the Health Sciences, South Glen Road 11455, Bethesda, 20854, Maryland, USA

    Johng S. Rhim

  2. , Department of Medicine, McGill University Health Centre, Pine Ave 687, Montreal, Québec, Canada

    Richard Kremer

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Lim, D.J., Moon, S.K. (2011). Establishment of Cell Lines from the Human Middle and Inner Ear Epithelial Cells. In: Rhim, J., Kremer, R. (eds) Human Cell Transformation. Advances in Experimental Medicine and Biology, vol 720. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0254-1_2

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