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Tyr8-Substance P Binding Study on a Plasma Membrane from Dog Ileum Smooth Muscle

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Substance P and Neurokinins

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Abstract

Binding of 125I-Tyr8-substance P (125I-Tyr8-SP) to a plasma membrane enriched fraction (PM) from the circular muscle of canine small intestine was studied. The binding was fast, reversible and saturable with a KD and Bmax of 0.5 ± 0.06 nm and 742 ± 173 fmol/mg protein, respectively. 125Ieledoisin did not bind to PM. These results together with competition studies suggest that PH possesses SP-P receptors but not SP-E receptors.

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Author information

Authors and Affiliations

  1. Department of Neurosciences, Faculty of Health Sciences, McMaster University, Hamilton, Ontario, Canada

    M. J. Muller, H. Sato & E. E. Daniel

Authors
  1. M. J. Muller
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  2. H. Sato
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  3. E. E. Daniel
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Editor information

Editors and Affiliations

  1. Department of Physiology, McGill University, Montreal, Quebec, H3G 1Y6, Canada

    James L. Henry Ph.D.

  2. Department of Physiology, University of Montreal, C.P. 6208, Montreal, Quebec, H3C 3T8, Canada

    Rejean Couture Ph.D.

  3. Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, H3G 1Y6, Canada

    A. Claudio Cuello M.D.

  4. MRC Group in Molecular Endocrinology, Centre Hospitalier de L’Universite, Laval, Quebec, G1V 4G2, Canada

    Georges Pelletier Ph.D.

  5. Douglas Hospital Research Centre and Department of Psychiatry, McGill University, Verdun, Quebec, H4H 1R3, Canada

    Remi Quirion Ph.D.

  6. Department of Pharmacology, Medical School, University of Sherbrooke, Sherbrooke, Quebec, J1H 5N4, Canada

    Domenico Regoli M.D.

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© 1987 Springer-Verlag New York Inc.

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Cite this paper

Muller, M.J., Sato, H., Daniel, E.E. (1987). Tyr8-Substance P Binding Study on a Plasma Membrane from Dog Ileum Smooth Muscle. In: Henry, J.L., Couture, R., Cuello, A.C., Pelletier, G., Quirion, R., Regoli, D. (eds) Substance P and Neurokinins. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-4672-5_27

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  • DOI: https://doi.org/10.1007/978-1-4612-4672-5_27

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-9109-1

  • Online ISBN: 978-1-4612-4672-5

  • eBook Packages: Springer Book Archive

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