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Simultaneous Determination of Ascorbic Acid and Dehydroascorbic Acid by High Performance Liquid Chromatography

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Abstract

It has been found that ascorbic acid (AA) and dehydro-ascorbic acid (DHAA) can be derivatized to the compounds having a maximum absorption wavelength of 3OOnm under alkaline conditions, and that der¡vatizati on of DHAA is accelerated in the presence of a reducing agent such as sodium borohydride. This reaction has been applied to post-column derivatization in high performance liquid chromatography (HPLC) for the simultaneous determination of AA and DHAA, In this system, AA in the range of 1ng 2µg and DHAA in the range of 2ng 2µg have been determined simultaneously with good reproducibility, C. V 1.5% (n = 10).

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Authors and Affiliations

  1. Analytical Applications Department, Shjmadzu Corporation, 1, Nishinokyo-kuwabaracho, Nakagyo-ku, Kyoto, Japan

    Yoshihide Yasui & Morimasa Hayashi

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  1. Yoshihide Yasui
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  2. Morimasa Hayashi
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Yasui, Y., Hayashi, M. Simultaneous Determination of Ascorbic Acid and Dehydroascorbic Acid by High Performance Liquid Chromatography. ANAL. SCI. 7 (Suppl 1), 125–128 (1991). https://doi.org/10.2116/analsci.7.Supple_125

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  • DOI: https://doi.org/10.2116/analsci.7.Supple_125

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