Abstract
Infectious diseases cause great economic loss and individual and even social anguish. Existing detection methods lack sensitivity and specificity, have a poor turnaround time, and are dependent on expensive equipment. In recent years, the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system has been widely used in the detection of pathogens that cause infectious diseases owing to its high specificity, sensitivity, and speed, and good accessibility. In this review, we discuss the discovery and development of the CRISPR-Cas system, summarize related analysis and interpretation methods, and discuss the existing applications of CRISPR-based detection of infectious pathogens using Cas proteins. We conclude the challenges and prospects of the CRISPR-Cas system in the detection of pathogens.
概要
感染性疾病对个人乃至社会均可造成极大的经济损失和精神痛苦。现有检测方法在灵敏度与特异性、检测时间、昂贵仪器设备依赖方面或多或少存在不足。成簇的规律间隔的短回文重复序列(CRISPR)-相关蛋白(Cas)系统,因其具备特异、灵敏、快速、便捷等优势,**年来被尝试应用于感染性疾病病原体的检测。本文首先回顾了CRISPR-Cas系统的发现发展进程,并列举可与之结合获得检测结果的检测结果阅读方式,之后从检测过程中所应用的不同Cas蛋白的角度简要描述了目前已有的基于CRISRP检测感染性疾病病原体的应用,最后还展望了CRISPR/Cas系统未来在检测方面的应用前景及挑战。
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This work was supported by the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2020JQ-148), the Guangdong Basic and Applied Basic Research Foundation (No. 2021A1515110793), and the Shenzhen Pathogen High-Throughput Sequencing Technical Engineering Laboratory Upgrading Project (No. 2019-986), China.
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Hongdan GAO prepared the manuscript of the article, including all figures and tables. Zifang SHANG completed the literature collection, the article writing, and the drawing of all the figures and tables. Siew Yin CHAN provided the proofreading of the article. Dongli MA provided guidance and suggestions for the article writing. All authors have read and approved the final version.
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Hongdan GAO, Zifang SHANG, Siew Yin CHAN, and Dongli MA declare that there are no conflicts of interest.
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Gao, H., Shang, Z., Chan, S.Y. et al. Recent advances in the use of the CRISPR-Cas system for the detection of infectious pathogens. J. Zhejiang Univ. Sci. B 23, 881–898 (2022). https://doi.org/10.1631/jzus.B2200068
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DOI: https://doi.org/10.1631/jzus.B2200068