Abstract
A field experiment was conducted to study the response of individual leaf spectral reflectance to five levels of nitrogen fertilizer treatments in rice (Oriza sativa L.) plants. Sampling was combined through a rice canopy at upper, medium and low levels for biomass, nitrogen and water content measurements with spectral signals from the leaves. The vertical gradients of leaf biomass, nitrogen and water contents were associated with the nitrogen availability during tillering, panicle formation, initial heading and heading. Rice plants treated with the lowest rate of N could be characterized with the lowest value of gradient in leaf biomass and leaf water content and the highest value of gradient in leaf N concentration. A spectral gradient of single reflectance (R), ratio (RVI) and normalized difference (ND) of two individual reflectances was defined as this yielded a better relationship between the spectral data and leaf nitrogen concentration. The results suggested the spectral gradients may be used as an improved diagnostic tool for nitrogen status.
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References
Al-Abbas A. H., Barr R., Hall J. D., et al., 1974. Spectra of normal and nutrient-deficient maize leaves.Agron. J. 66:16–20.
Blackner, T. M., Schepers, J. S. and Varvel, G. E., 1994. Light reflectance compared to other N stress measurements in corn leaves.Agron. J. 86:934–938.
Blackner, T. M., Schepers, J. S., Varvel, G. E., et al., 1996. Nitrogen deficiency detection using reflected shortware radiation from irrigated corn canopies.Agron. J. 88:1–5.
Carter, G. A., 1993. Responses of leaf spectral reflectance to plant stress.Am. J. Bot. 80:239–243.
Drouet, J-L., and Bonhomme, R., 1999. Do variations in local leaf irradiance explain changes to leaf nitrogen within row maize canopies?.Annals of Botany 84:61–69.
Filella, I., Serrano, L., Serra, J., et al., 1995. Evaluating wheat nitrogen status with canopy reflectance indices and discriminant analysis.Crop Sci.,35:1400–1405.
Guyot, G., 1990. Optical properties of vegetative canopies. p. 19–44.In: Application of remote sensing in agriculture. M D Steven and J A Clark Eds. Butterworths Publ., London, p. 19–44.
Hinzman, L. D., Bauer, M. E. and Daughity, C. S. T., 1986. Effects of nitrogen fertilization on growth and reflectance characteristics of winter wheat.Remote Sens. Environ. 19:47–61.
Ladha, J. K., Tirol-Padre, A., Punzalan, G. C., et al., 1998. Nondestructive estimation of shoot nitrogen in different rice genotypes.Agron. J. 90:33–40.
Liu, Z. Y., 1982. A Collection of Illustrative Plates for Nutritional Disorder in Principal Crops in China. Agricultural Publishing Press, Bei**g (in Chinese).
Riedell, W. E., Blackmer, T. M., 1999. Leaf reflectance spectra of cereal aphid-damaged wheat.Crop Sci. 39:1835–1840.
Ruano-Ramos, A., García-Ciadad, A., García-Criado, B., 1999. Determination of nitrogen and ash contents in total herbage and botanical components of grassland systems with near infra-red spectroscopy.J. Sci. Food Agric. 79:137–143.
Scaife, A., 1988. Derivation of critical nutrient concentrations for growth rate from data from field experiments.Plant and Soil. 109:159–169.
Smith, F. W., 1986 Interpretation of plant analysis: concepts and principles.In: Plant Analysis: an Interpretation Manual. D J Reuter and J B Robinson Eds. Inkata Press, Melbourne.
Takebe, M., Yoneyama, T., Inada, K., et al., 1990. Spectral reflectance ratio of rice canopy for estimating crop nitrogen status.Plant and Soil. 122:295–297.
Thomas, J. R., and Oerther, G. F., 1972. Estimating nitrogen content of sweet pepper leaves by reflectance measurements.Agron. J. 64:11–13.
Walburg, G., Bauer, M. E., Daughtry, C. S. T., et al., 1982. Effects of nitrogen nutrition on the growth, yield, and reflectance characteristics of corn canopies.Agron. J. 74:677–683.
Wang, R. C., Wang, K., Shen, Z. Q., 1998. Feasibility of field evaluation of rice nitrogen status from reflectance spectra of canopy.Pedosphere 8:121–126.
Zhou, Q. F., and Wang, R. C., 1993. The relation between leaf spectral characteristics and nitrogen level in early rice.J. Zhejiang Agric. Univ. 19(Sup):15–22.
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Project (49771056) supported by Natural Science Foundation of China(NSFC).
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Qi-fa, Z., Ren-chao, W., Zhang-quan, S. et al. Vertical leaf spectral variation as an indicator of nitrogen nutrition status in rice plants. J. Zhejiang Univ.-Sci. 2, 289–293 (2001). https://doi.org/10.1631/BF02839462
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DOI: https://doi.org/10.1631/BF02839462