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Pathological and molecular identification of porcine cysticercosis in Maharashtra, India

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Abstract

Porcine cysticercosis, caused by metacestodes of Taenia solium is an important emerging zoonotic disease with public health and economic significance. Pigs acquire the disease through consumption of Taenia solium eggs excreted by human tapeworm carriers. The present study was conducted to investigate the prevalence of porcine cysticercosis in Nagpur and Mumbai region of Maharashtra, India by P/M examination of carcasses followed by histopathology of affected organs in infected animals and molecular identification of cysts for confirmation. Out of 1000 pigs examined during slaughter, three pigs were found to be heavily affected with T. solium cysts giving a prevalence of 0.3%. Histological section of brain in infected animals revealed marked vascular congestion of meninges, mild neuronal degeneration, perivascular cuffing and gliosis while the liver showed the infiltration of mononuclear cell, predominantly eosinophils throughout the parenchyma. Some degree of calcification was observed in the cysts lodged in liver while calcification was not evident in case of cysts lodged in brain, tongue, diaphragm and skeletal muscle. Molecular identification by PCR using two sets of oligonucleotide primers against LSU rRNA gene and Mt-Cox1 gene of T. solium confirms the cysts to be that of T. solium. The molecular diagnostics methods have been considered for validation in conjunction with P/M inspections, parasitological and histopathological examinations. The study confirms the presence of porcine cysticercosis in the two regions and demands proper sanitary measures to minimize the risk of infection from zoonoses and food safety point of view.

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Authors and Affiliations

  1. Department of Veterinary Public Health, Nagpur Veterinary College, Nagpur, Maharashtra, 440006, India

    Kaushik Satyaprakash, Waqar A. Khan, Sandeep P. Chaudhari & Shilpshree V. Shinde

  2. Department of Veterinary Pathology, Nagpur Veterinary College, Nagpur, Maharashtra, 440006, India

    Nitin V. Kurkure

  3. Department of Veterinary Parasitology, Nagpur Veterinary College, Nagpur, Maharashtra, 440006, India

    Sunil W. Kolte

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  1. Kaushik Satyaprakash
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  2. Waqar A. Khan
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  3. Sandeep P. Chaudhari
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  4. Shilpshree V. Shinde
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  5. Nitin V. Kurkure
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Correspondence to Kaushik Satyaprakash.

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Satyaprakash, K., Khan, W.A., Chaudhari, S.P. et al. Pathological and molecular identification of porcine cysticercosis in Maharashtra, India. Acta Parasit. 63, 784–790 (2018). https://doi.org/10.1515/ap-2018-0094

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