Abstract
Antibodies (ab) in the serum of allograft recipients against donor lymphocytes (L) are associated with graft rejection. Because ab may be produced against B cell (BC) antigens (ag) as well as HLA ag, we prospectively evaluated the sera of 27 pediatric allograft recipients for ab against isolated T and B donor L by the microcytotoxicity crossmatch test (XM) and correlated results with graft outcome. All patients had negative TC XMs. 16 had positive (+) BC XMs, while 11 had negative (-) BC XMs. 8 grafts failed in 2 weeks to 6 months; 3 with + and 5 with - BC XMs. A serum creatinine level of <1.5 mg/dl was present at 1 month in 9 of 16 BC XM + patients (56%) vs 2 of 11 BC XM - (18%); at 3 months in 9 of 14 + BC XM (64%) vs 4 of 11 BC XM -(36%); at 6 months in 8 of 11 BC XM + (72%) vs 4 of 10 BC XM - (40%); and at 9 months in 6 of 10 BC XM + (60%) vs 2 of 8 BC XM - (25%). No statistically significant difference was present at any time interval. The sera of these 11 patients and 15 additional adult graft recipients were studied for blocking-factors in the one-way Mixed Lymphocyte Culture(MLC) (donor-stimulator;recipient-responder). Of 10 BC XM + sera, 9 (90%) showed MLC blocking; of 16 BC XM - sera, 3(19%) showed blocking (p<.01). These data indicate that ab against donor BC specificities are not deleterious to allograft function and may be directed against MLC stimulatory ag or ag in close proximity present on the B lymphocyte.
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Ettenger, R., Terasaki, P., Maiedzadeh, M. et al. ANTIBODIES TO B-LYMPHOCYTES IN RENAL TRANSPLANTATION. Pediatr Res 11, 550 (1977). https://doi.org/10.1203/00006450-197704000-01079
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DOI: https://doi.org/10.1203/00006450-197704000-01079
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