Background

Enterotoxigenic E. coli (ETEC) strains expressing F4 or F18 fimbriae are major causes of post-weaning diarrhea in nursery pigs [1]. Attachment of ETEC to the specific receptors on intestinal epithelium leads to colonization and secretion of enterotoxins, resulting in secretory diarrhea in weanling pigs [2]. To prevent post-weaning diarrhea and improve production of pigs, antibiotics were commonly added to the diet over the past decades. However, frequent use of in-feed antibiotics in livestock production has been shown to contribute to the increased prevalence of antibiotic-resistant bacteria and raised public health concerns [3]. With these issues, the Food and Drug Administration (FDA) banned the use of in-feed antibiotics for growth promoting purposes in livestock production in the U.S. [4], thus alternative nutritional strategies are highly demanded to enhance disease resistance and production of weanling pigs.

Many nutritional approaches have been applied to prevent post-weaning diarrhea associated with ETEC and enhance the production of pigs. Among these, the prevention of bacterial attachment to the small intestine is one of the most effective defense strategies against ETEC infection [5]. Oligosaccharides have been reported to possess ETEC receptor activity for bacterial adhesions [6, 7]. Especially, N-acetylgalactosamine (GalNAc) containing glycans that could enhance the binding affinity of various ETEC strains, including K99 [8], F4 [9], and F18 [10,11,12]. Coddens et al. [11] identified the blood group H type 1 determinant (Fuca2Galß3GlcNAc) as the minimal binding epitope of F18 fimbriae. Based on that, an optimal binding epitope was created by adding the terminal 3-linked galactose or N-acetylgalactosamine of the blood group B type 1 determinant (Galα3(Fucα2)Galß3GlcNAc) and the blood group A type 1 determinant (GalNAcα3(Fucα2)- Galß3GlcNAc). The purified soluble blood group oligosaccharides were able to greatly reduce binding of F18 positive E. coli to intestinal villi of F18 receptor-positive pigs in concentrations of 1 to 10 mg/mL [11]. Moreover, multimerizing blood group A on human serum albumin reduced the amount of blood group oligosaccharides needed 1000 times [13]. Therefore, multimerizing the blood group A oligosaccharides may efficiently prevent enterotoxin-induced secretory diarrhea. Recently, grafted polymers that combine multiple substances have been proposed for their potential synergistic effects on preventing human and animal diseases [14, 15]. Epsilon-poly-lysin (ε-PL) has been reported to use as a carrier in the membrane transport of proteins and drugs [16]. Due to its excellent heat stability, biodegradability, and lack of toxicity, ε-PL has generally been regarded as safe status (GRAS) and been interested in the food and medicine industries as a delivery vehicle targeting the desired location [17]. Thus, the combination of blood group oligosaccharides and ε-PL may enhance the resistance of pigs against F18 ETEC infection by inhibiting bacterial attachment and/or directly killing bacteria. The overall objective of this study was to investigate the efficacy of blood group A6 type 1-based polymer on gut integrity and disease resistance of weanling pigs challenged with F18 ETEC.

Materials and methods

Animals, housing, experimental design, and diet

A total of 48 weanling pigs (crossbred; initial body weight (BW): 7.23 ± 1.14 kg; 21 days old) with an equal number of gilts and barrows were used in this study. They were selected from the Swine Teaching and Research Center at the University of California, Davis. The sows and piglets used in this experiment did not receive E. coli vaccines, antibiotic injections, or antibiotics in creep feed. Before weaning, feces were collected from sows and all their piglets destined for this study to verify the absence of β-hemolytic E. coli. The F18 ETEC receptor status was also tested based on the methods of Kreuzer et al. [18], and all piglets used in this study were susceptible to F18 ETEC.

After weaning, all pigs were randomly assigned to one of the four dietary treatments in a randomized complete block design with body weight within sex and litter as the blocks and pig as the experimental unit. There were 12 replicate pigs per treatment. Pigs were individually housed (pen size: 0.61m × 1.22 m) in environmental control rooms at the Cole Facility at the University of California, Davis for 18 d, including 7 d before and 11 d after the first F18 ETEC challenge (d 0). The piglets had ad libitum access to feed and water. Environmental enrichment was provided for each pig. The light was on at 07:00 h and off at 19:00 h daily in the environmental control rooms.

The 4 dietary treatments included: 1) Positive control: control diet; 2) Low dose oligosaccharide-based polymer (LOW): control diet supplemented with 10 mg/kg oligosaccharide-based polymer active substance (Coligo); 3) High dose oligosaccharide-based polymer (HIGH): control diet supplemented with 20 mg/kg oligosaccharide-based polymer active substance (Coligo); and 4) CAR: control diet supplemented with 50 mg/kg carbadox. Spray-dried plasma and high levels of zinc oxide exceeding recommendation and normal practice were not included in the diets. The experimental diets were fed to pigs throughout the experiment. Oligosaccharide-based polymer active substance was a glycoconjugate composed of blood group A6 type 1 antigen oligosaccharides grafted on a single peptide of epsilon-poly-lysine. Coligo was designed and synthesized by Elicityl (France) in cooperation with Ghent University (Dr. Eric Cox’s laboratory) and was provided by Pancosma (Geneva, Switzerland). The mean rate of conjugation is 15 mol of oligosaccharide for 1 mol of ε-PL. The oligosaccharide part represents 25% of the molecular weight of the oligosaccharide-based polymer. All diets were formulated to meet pig nutritional requirements (Table 1) [19] and provided as mash form throughout the experiment.

Table 1 Ingredient compositions of experimental diets1
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After 7 days of adaptation, all pigs were orally inoculated with 3 mL of F18 ETEC for 3 consecutive days from d 0 post-inoculation (PI). The F18 ETEC was originally isolated from a field disease outbreak by the University of Illinois Veterinary Diagnostic Lab (isolate number: U.IL-VDL # 05–27,242). The F18 ETEC expresses heat-labile toxin (LT), heat-stable toxin b (STb), and Shiga-like toxin (Stx2e). The inoculums were prepared by the laboratory of the Western Institute for Food Safety and Security at the University of California, Davis, and were provided at 1010 colony-forming unit (CFU) per 3 mL dose in phosphate-buffered saline (PBS). This dose caused mild diarrhea in the current study, consistent with our previous published researches [20,21,1). The qRT-PCR reaction conditions followed the published research [27]. The 2-ΔΔCT method was used to analyze the relative expression of genes compared with control [28].

Statistical analysis

The normality of data was verified with the Shapiro-Wilk test, and outliers were identified using the UNIVARIATE procedure (SAS Inst. Inc., Cary, NC, USA). All data were analyzed by ANOVA using the PROC MIXED of SAS (SAS Institute Inc., Cary, NC, USA) in a randomized complete block design with the pig as the experimental unit. The statistical model included independent variables treatment group, sampling day, and interactions as the fixed effect and blocks as random effects. Treatment means were separated by using the LSMEANS statement and the PDIFF option of PROC MIXED. Contrast statements were used to analyze the dose effects of Coligo. The Chi-square test was used for analyzing the frequency of diarrhea. Statistical significance and tendency were considered at P < 0.05 and 0.05 ≤ P < 0.10, respectively.

Results

Growth performance, diarrhea score, β-hemolytic coliforms

No difference was observed in the initial BW and d 0 BW of pigs among dietary treatments (Table 2). Pigs supplemented with CAR had greater (P < 0.05) BW on d 5 PI than pigs in the control and HIGH groups. Pigs supplemented with LOW had the greatest (P < 0.05) BW, but pigs supplemented with HIGH had the lowest (P < 0.05) BW on d 11 PI among all dietary treatments. Supplementation of LOW had greater (P < 0.05) ADFI of pigs from d 5 to 11 PI, compared with control and HIGH groups. Supplementation of Coligo had greater (P < 0.05) feed efficiency from d 0 to 5 PI compared with pigs in the control group regardless of dose. Supplementation of HIGH also had greater (P < 0.05) feed efficiency of weaned pigs from d 5 to 11 PI, compared with pigs in the control. Pigs fed with CAR had better (P < 0.05) feed efficiency than pigs fed with the control diet from d 0 to 5 PI, but this was not the case from d 5 to 11 PI.

Table 2 Growth performance of ETEC-infected pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics
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Pigs supplemented with CAR had the lowest (P < 0.05) average diarrhea score from d 0 to 5 PI and d 5 to 11 PI among all dietary treatments (Table 3; Fig. 1). Compared with pigs in control group, pigs supplemented with LOW had lower (P < 0.05) average diarrhea score of weaned pigs from d 0 to 5 PI, but this was not the case from d 5 to 11 PI. Supplementation of CAR or any dose of Coligo had lower (P < 0.05) frequency of diarrhea of weaned pigs from d 0 to 11 PI.

Table 3 Diarrhea score and frequency of diarrhea of ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics
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Fig. 1
figure 1

Daily diarrhea score of ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics. Diarrhea score = 1, normal feces, 2, moist feces, 3, mild diarrhea, 4, severe diarrhea, 5, watery diarrhea. Each least squares mean from d 0 to d 5 post-inoculation (PI) represents 12 observations. Each least squares mean from d 6 to d 11 PI represents 6 observations. *Significant differences were observed among dietary treatment: P < 0.05. LOW = Low dose blood group A6 type 1-based polymer (Coligo); HIGH = High dose blood group A6 type 1-based polymer (Coligo); CAR = Carbadox

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No β-hemolytic coliform was observed in the feces of all pigs before ETEC inoculation. Pigs supplemented with CAR had the lowest (P < 0.05) β-hemolytic coliform percentage in feces on d 2 and 5 PI among all dietary treatments (Fig. 2). The percentage of β-hemolytic coliform in feces was not different between Coligo groups and CAR on d 8 PI. There were no differences observed in fecal culture on d 11 PI among the treatments.

Fig. 2
figure 2

The percentage (%) of β-hemolytic coliform in fecal samples of ETEC-infected pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics. Each least squares mean from d 0 to d 5 post-inoculation (PI) represents 12 observations. Each least squares mean from d 6 to d 11 PI represents 6 observations. a,bMeans without a common superscript differ (P < 0.05). LOW = Low dose blood group A6 type 1-based polymer (Coligo); HIGH = High dose blood group A6 type 1-based polymer (Coligo); CAR = Carbadox

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Systemic immunity and red blood cell profile

Lymphocyte counts were greater (P < 0.05) in pigs fed CAR on d 0 before ETEC inoculation (Table 4). Pigs in the LOW group had lower (P < 0.05) neutrophils, lymphocytes, and basophils on d 2 PI and lower (P < 0.05) neutrophil counts on d 5 PI, compared with pigs in the control group. Supplementation of HIGH also had lower (P < 0.05) white blood cell counts, neutrophils, lymphocytes, and basophils on d 2 PI. Pigs in the CAR group had lower (P < 0.05) neutrophils and basophils on d 2 PI and lower (P < 0.05) neutrophils on d 5 PI, but higher (P < 0.05) eosinophils on d 5 PI, compared with pigs in control group. No difference was observed in white blood cell profiles among treatments on d 11 PI.

Table 4 Total and differential white blood cells, and serum cytokine and acute-phase proteins in ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics
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No difference was observed in serum TNF-α concentration among dietary treatments throughout the experiment. Compared with the pigs fed control diet, pigs supplemented with LOW had lower (P < 0.05) haptoglobin on d 5 PI, while pigs fed CAR had lower (P < 0.05) C-reactive protein on d 2, 5, and 11 PI and had lower (P < 0.05) haptoglobin on d 5 PI. No differences in serum C-reactive protein and haptoglobin were observed between the control and HIGH groups.

Before ETEC inoculation, pigs in the CAR group had the lowest (P < 0.05) mean corpuscular volume and total platelets among all dietary treatments on d 0 (Table S2). Supplementation of LOW had lower (P < 0.05) red blood cells and packed cell volume on d 2 PI, while supplementation of HIGH had lower (P < 0.05) packed cell volume on d 5 PI, compared with pigs in the control. Pigs supplemented with CAR had lower (P < 0.05) red blood cells and packed cell volume, but higher (P < 0.05) mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration on d 2 and 5 PI, compared with pigs in the control. Supplementation of CAR also had greater (P < 0.05) total protein concentration on d 11 PI in comparison to pigs in the other treatments.

Bacterial translocation

Supplementation of HIGH had lower (P < 0.05) bacterial translocation in lymph nodes on d 5 and 11 PI compared with control group (Fig. 3). Pigs supplemented with Coligo or CAR had lower (P < 0.05) bacterial translocation in the spleen than pigs in the control on d 11 PI.

Fig. 3
figure 3

Bacterial counts (CFU/g) in lymph node and spleen of ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics. Each least squares mean from d 0 to d 5 post-inoculation (PI) represents 12 observations. Each least squares mean from d 6 to d 11 PI represents 6 observations. a,bMeans without a common superscript differ (P < 0.05). LOW = Low dose blood group A6 type 1-based polymer (Coligo); HIGH = High dose blood group A6 type 1-based polymer (Coligo); CAR = Carbadox

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Intestinal morphology

On d 5 PI, supplementation of Coligo dose-dependently had greater (linear, P < 0.05) villi height, the ratio of villi height to crypt depth, villi width, and villi area in duodenum, had greater (linear, P < 0.05) the ratio of villi height to crypt depth in jejunum, and had greater (linear, P < 0.05) villi height, the ratio of villi height to crypt depth, and villi area in ileum, compared with the control group (Table S3). Supplementation of Coligo also had greater (linear, P < 0.05) duodenal and jejunal villi height and jejunal and ileal villi area, and tended to have greater (linear, P < 0.10) the ratio of villi height to crypt depth in jejunum and ileal villi height on d 11 PI. Pigs fed with CAR had greater (P < 0.05) villi height in duodenum and ileum, the ratio of villi height to crypt depth in all three intestinal segments, and villi area in duodenum than pigs in the control group on d 5 PI. On d 11 PI, pigs supplemented with CAR had higher (P < 0.05) villi height in all three intestinal segments, greater (P < 0.05) villi height to crypt depth ratio in jejunum, and bigger (P < 0.05) sialomucin area in duodenum than pigs in the control group. In addition, pigs in the CAR group also had greater (P < 0.05) villi height:crypt depth in all intestinal segments on d 5 PI, and greater (P < 0.05) villi height in ileum, in comparison to pigs in the Coligo treatment group.

Intestinal barrier and innate immunity

No difference was observed in the mRNA expression of MUC2 in jejunal mucosa among pigs in all dietary treatment groups (Fig.4). On d 5 PI, supplementation of HIGH up-regulated (P < 0.05) the mRNA expression of ZO1 and addition of CAR had greater (P < 0.05) mRNA expression of OCLN, compared with pigs in control group. On d 11 PI, supplementation of LOW or CAR had higher (P < 0.05) mRNA expression of CLDN1 in jejunal mucosa of weaned pigs, compared with the control and HIGH groups. On d 5 PI, supplementation of LOW down-regulated (P < 0.05) the mRNA expression of IL6, supplementation of HIGH had lower (P < 0.05) mRNA expression of IL1B, IL6, and TNF, and supplementation of CAR had lower (P < 0.05) IL1B and IL6 gene expression in ileal mucosa of weaned pigs in comparison to control pigs (Fig. 5). Supplementation of HIGH also had lower (P < 0.05) IL6 mRNA expression on d 11 PI in ileal mucosa, compared with the control group. However, no differences were observed in the gene expression of inflammatory mediators among LOW, HIGH, and CAR groups.

Fig. 4
figure 4

Gene expression profiles in jejunal mucosa of ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics on d 5 or 11 post-inoculation (PI). a,bMeans without a common superscript differ (P < 0.05). Each least squares mean represents 6 observations. LOW = Low dose blood group A6 type 1-based polymer (Coligo); HIGH = High dose blood group A6 type 1-based polymer (Coligo); CAR = Carbadox; MUC2 = Mucin-2; CLDN1 = Claudin-1; ZO-1 = Zonula occludens-1; OCDN = Occludin

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Fig. 5
figure 5

Gene expression profiles in ileal mucosa of ETEC-infected weaned pigs fed diets supplemented with oligosaccharide-based polymer (Coligo) or antibiotics on d 5 or 11 post-inoculation (PI). a,bMeans without a common superscript differ (P < 0.05). Each least squares mean represents 6 observations. LOW = Low dose blood group A6 type 1-based polymer (Coligo); HIGH = High dose blood group A6 type 1-based polymer (Coligo); CAR = Carbadox; IL1B: Interleukin-1 beta; IL6: Interleukin-6; TNF = Tumor necrosis factor; PTGS2: Cyclooxygenase-2

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Discussion

ETEC infection is initiated by bacterial attachment to specific receptors on the intestinal epithelium by fimbrial adhesins, followed by colonization of ETEC in the small intestine [29]. Once colonization is established, ETEC rapidly proliferate and produce one or more enterotoxins, which can stimulate water and electrolyte secretion and reduce fluid absorption in the small intestine and induce diarrhea [30]. Diarrhea caused by ETEC is one of the most prevalent diseases during the weaning stage, which is responsible for anorexia, slower growth, or even the death of pigs. Results of the present study demonstrated that supplementation of Coligo improved growth rate, and reduced frequency of diarrhea and systemic inflammation of weaned pigs experimentally challenged with F18 ETEC. The potential mechanisms of action include inhibition of binding of bacteria and as such colonization of the gut by the F18 ETEC [11, 13], enhancing gut barrier function and reducing local and systemic inflammation.

In the current study, pigs in the control group grew slower and had a high frequency of diarrhea compared to pigs without ETEC challenge in our previous research [20, 56] and Priori et al. [57] suggest changes in the intestinal microbiota are affected by porcine blood group. Thus, the blood group A antigen in Coligo may affect the composition and function of microbial communities when fed to pigs. Moreover, recent studies demonstrated that dietary supplementation of ε-PL altered ileal microbiota structure and function in pigs [58] and fecal microbial community in mice [59]. ε-PL supplementation may promote the growth of beneficial microorganisms in the intestinal tract, therefore, reducing the proliferation of pathogens. The exact mechanisms of ε-PL in the current study remain unclear, so further research is needed to confirm the effects of Coligo on the pigs’ gut microbial community, intestinal inflammation, and immune responses against F18 ETEC. Pigs supplemented antibiotics also had reductions in mRNA expression of proinflammatory markers in the present study. This finding demonstrated that pigs supplemented antibiotics has less severe intestinal inflammation than pigs in control group. In agreement with previous research, antibiotic supplements might exert anti-inflammatory properties in the intestine or accumulate in phagocytic inflammatory cells, therefore, attenuating inflammatory responses in animals [60, 61]. Taken altogether, down-regulation in mRNA expression of proinflammatory cytokines by Coligo or antibiotics supplementation is beneficial for pigs in terms of their intestinal health and growth performance.

In conclusion, results in the current study suggest that in-feed supplementation of Coligo or antibiotic (carbadox) enhanced growth performance and reduced the severity of diarrhea caused by ETEC F18 infection. Although the percentage of β-hemolytic coliforms in fecal samples of pigs fed with Coligo was less diminished than pigs supplemented with antibiotics, enhanced disease resistance was demonstrated by the improved gut barrier integrity and attenuated systemic and intestinal inflammation. To further explore the mechanisms of action of Coligo, integrated metabolomics and metagenomics approaches may be considered to provide more insights into the beneficial effects of Coligo or other polymers on pigs’ health. Overall, the current study indicates that supplementation with Coligo has promising impacts on promoting growth and disease resistance of newly weaned pigs infected with ETEC F18. The efficacy of Coligo is comparable to antibiotic (carbadox) demonstrating the potential of Coligo as antibiotic alternative for animal growth performance and disease resistance. Large-scale animal trials are recommended to further evaluate the impacts of Coligo on performance of weaned pigs under commercial practice conditions.