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RNA-Seq-Based Transcriptome Analysis of Changes in Gene Expression Linked to Human Pregnancy Outcome After In Vitro Fertilization—Embryo Transfer

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Abstract

To promote the pregnancy rate of in vitro fertilization–embryo transfer (IVF-ET), we proposed to examine the differentially expressed gene during pregnancy success and failure. We used high-sequencing technology to characterize and compare the gene expression profiles of pregnancy success and pregnancy failure patients during 3 different stages: before IVF-ET (stage I), after ovarian stimulation (stage II), and day 15 after embryo transfer (stage III). Selected data from RNA-sequencing experiments were validated by quantitative real-time polymerase chain reaction (qRT-PCR). A total of 282, 208, and 372 genes in stages I, II, and III, respectively, were differentially expressed between pregnancy success and pregnancy failure, respectively. Through confirmation with qRT-PCR, compared to pregnancy failure, we demonstrated much lower level of major histocompatibility complex, human leukocyte antigen class I A, and an much higher level of human leukocyte antigen, class II DQ α1 (HLA-DQA1) in pregnancy success, although the HLA-DQA1 decreased with development duration of pregnancy. Interleukin 1β increased with the development duration of pregnancy in pregnancy failure group and was much higher than that in pregnancy success group. Hemoglobin δ decreased with the development duration of pregnancy in pregnancy failure women and maintained in a lower level in stage I and II but dramatically increased to a much higher level in stage III in pregnancy success women. Minichromosome maintenance complex component 4 significantly increased in stage III in pregnancy failure but not in pregnancy success women. The altered expression of genes implicated in immune response and inflammation, oocyte meiosis, rhythmic process, and so on. Therefore, the current results provide a strong basis for future research to expound the molecular mechanism co** with pregnancy outcome.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shenzhen Maternal and Child Health Care Hospital, Affiliated to Southern Medical University, 518028, Shenzhen, People’s Republic of China

    Ruopeng Zhang MD & Chenhong Wang MD

  2. Graduate School, Southern Medical University, Guangzhou, People’s Republic of China

    Ruopeng Zhang MD

  3. Department of Reproductive Medicine, Affiliated hospital of Dali University, Dali, People’s Republic of China

    Chenghe Yu BS, Rui Wu BS, Lirong Zhang BS, Li Zhu BS & Anli Xu BS

Authors
  1. Ruopeng Zhang MD
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  2. Chenghe Yu BS
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  3. Rui Wu BS
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  4. Lirong Zhang BS
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  5. Li Zhu BS
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  7. Chenhong Wang MD
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Correspondence to Chenhong Wang MD.

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Zhang, R., Yu, C., Wu, R. et al. RNA-Seq-Based Transcriptome Analysis of Changes in Gene Expression Linked to Human Pregnancy Outcome After In Vitro Fertilization—Embryo Transfer. Reprod. Sci. 23, 134–145 (2016). https://doi.org/10.1177/1933719115597766

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