Abstract
A biodegradable tubular poly(L-lactide) scaffold and a method for seeding and culturing mesenchymal stem cells were developed to create a tissue-engineered vascular graft. In group 1, scaffolds without pre-seeded cells were implanted into the rat abdominal aorta; in group 2, scaffolds with pre-seeded adipose-derived mesenchymal stem cells were used. The follow-up period in group 1 ranged from 2 days to 64 weeks (n = 36), while this time was up to 72 weeks in group 2 (n = 42). The graft patency in group 1 was 86%; in group 2 - 97%. Histology revealed a gradual biodegradation of the biopolymer and the replacement of the polymer fibers with connective tissue in the group 1. However, after 64 weeks complete polymer biodegradation was achieved, and in all cases, the formation of graft aneurysms was detected. In group 2, the total cells number in the graft and the thickness of the neoadventitia around it, were significantly higher, however, the grafts morphology at the late follow-up did not qualitatively differ in four out of nine cases (45%), a graft aneurysm developed . Thus, the preliminary cultivation of mesenchymal stemcells on the scaffold provoked a pronounced host cellular reaction, which probably made it possible to reduce the incidence of aneurysm formation, but did not ensure the formation of a natural vessel structure.
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This work was supported by the Russian Science Foundation, project no. 19-73-30003.
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Statement on the welfare of animals. Animal experiments were performed in accordance with the rules for the use of experimental animals (European Convention, Strasbourg, 1986 World Medical Association and Declaration of Helsinki on Humane Treatment of Animals, 1996).
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Translated by I. Fridlyanskaya
Abbreviations: MFBGC—multinucleated foreign-body giant cell; MSC-AT—adipose-derived mesenchymal stem cells (MSC); PLLA—poly(L-lactide).
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Popov, G.I., Vavilov, V.N., Popryadukhin, P.V. et al. Assessment of a Tissue-Engineered Vascular Graft Based on a Biodegradable Scaffold and Mesenchymal Stem Cells in a Long-Term Experiment In Vivo. Cell Tiss. Biol. 17, 188–196 (2023). https://doi.org/10.1134/S1990519X23020116
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DOI: https://doi.org/10.1134/S1990519X23020116