Abstract
This work is aimed at studying the mechanisms of reciprocal humoral regulation of noradrenaline-producing organs in rats in the perinatal period of development. The activity of noradrenaline synthesis enzymes tyrosine hydroxylase and dopamine-beta-hydroxylase was measured in the brain and adrenal glands 48 and 72 h after the injection of immunotoxin (anti-dopamine-beta-hydroxylase-saporin) into the rat brain ventricles. It was shown that, 48 h after the immunotoxin injection into the brain, the activity of tyrosine hydroxylase in the brain decreased; however, 72 h after the injection it reached the control levels. This fact indicates that noradrenaline synthesis in the survived neurons increases. In the adrenal glands, 72 h after the immunotoxin injection into the brain, the activity of dopamine-beta-hydroxylase increased. This points to a compensatory increase in the rate of noradrenaline synthesis in the adrenal glands when the synthesis of noradrenaline in the brain is inhibited.
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Funding
This work was supported by the Russian Foundation for Basic Research (project no. 18-34-00929 (experiments on determination of the activity of TH and DBH 72 h after the injection of anti-DBH- saporin into the brain)) and the Russian Science Foundation (project no. 14-15-01122 (experiments on determination of the activity of TH and DBH 48 h after the injection of anti-DBH- saporin into the brain)).
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Statement on the welfare of animals. All manipulations with animals were carried out according to the protocol approved by the Commission on Bioethics of the Koltzov Institute of Development Biology, Russian Academy of Sciences, and were in accordance with national and international requirements.
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Translated by M. Batrukova
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Murtazina, A.R., Nikishina, Y.O. & Ugrumov, M.V. The Role of the Brain in the Regulation of Peripheral Organs–Noradrenaline Sources in Neonatal Rats: Noradrenaline Synthesis Enzyme Activity. Dokl Biochem Biophys 493, 201–204 (2020). https://doi.org/10.1134/S1607672920040109
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DOI: https://doi.org/10.1134/S1607672920040109