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Effects of 1,25(OH)2D3 on the Growth and Development of Skin Hair Follicles of Liaoning Cashmere Goat

  • CELL BIOLOGY
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Abstract

The Liaoning cashmere goat is a unique breed in China. It has an excellent germplasm resource with high villus yield, fine villus fibre quality, stable genetic performance and significant economic benefits.In this study, the effects of different concentrations of 1.25(OH)2D3 on the proliferation of skin fibroblasts was detected via CCK8 assay, while the expression of genes and proteins related to villus growth in Liaoning cashmere goat were detected by qRT-PCR and Western Blot. Treatment of Liaoning cashmere goat skin fibroblasts with 0.01 ng/L of 1.25(OH)2D3 for 72 h significantly promoted the proliferation of Liaoning cashmere goat skin fibroblasts, increased the expression of VDR, WNT10B, LEF1 and β-catenin genes, and decreased the expression of BMP4 and BMPRIB genes, indicating that 1.25(OH)2D3 plays a positive role in regulating hair follicle growth and development through the WNT and BMP signaling pathway, which positively regulates the growth and development of hair follicles. VDR interacting proteins were screened using Co-IP combined mass spectrometry. After treatment of cells with 0.01 ng/L of 1.25(OH)2D3 for 72 h, VDR interacting proteins were enriched to PI3K-AKT signaling pathways associated with hair follicle growth and development. Moreover, mitogen-activated protein kinase, protein S100, protein disulfide bond isomerase protein, FLII actin remodeling protein and multifunctional proteoglycan protein related to the growth and development of hair follicles were upregulated, indicating that a positive regulatory effect of 1.25(OH)2D3 on hair follicle growth and development.

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Funding

This work was supported by Natural Science Foundation of China (no. 31772557).

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Authors and Affiliations

  1. Department of Life Sciences, Liaoning Normal University, 116029, Dalian, China

    Mei **, Yan Zhang, Yunpeng Lu, **g’ai Piao, Jun Piao & Fengqin Zhao

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  1. Mei **
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  2. Yan Zhang
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  3. Yunpeng Lu
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  4. **g’ai Piao
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  5. Jun Piao
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  6. Fengqin Zhao
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Mei **, Yan Zhang, Yunpeng Lu, **g’ai Piao, Jun Piao and Fengqin Zhao. The first draft of the manuscript was written by Mei ** and all authors commented on previous versions of the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Mei **.

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Conflict of interest. The authors declare that they have no conflicts of interest.

Statement on the welfare of animals. This study was carried out in the Liaoning Province, Department of Life Sciences, Liaoning Provincial Key Laboratory of Biotechnology and Molecular Drug Development. All animal experiments were approved by the Animal Protection and Use Committee of the Department of Life Sciences of Liaoning Normal University and approved by the Chinese Agriculture Committee. All handling of Liaoning cashmere goats was carried out in accordance with the guidelines for animal protection and treatment systems.

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Mei **, Zhang, Y., Lu, Y. et al. Effects of 1,25(OH)2D3 on the Growth and Development of Skin Hair Follicles of Liaoning Cashmere Goat. Biol Bull Russ Acad Sci 50, 489–499 (2023). https://doi.org/10.1134/S1062359022602555

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