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Molecular Marking in Brassica oleracea L. Breeding for Resistance to Fusarium Wilt

  • PLANT GENETICS
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Abstract

This article presents the results of research on the identification of informative DNA marker systems providing reliable control of the presence of the resistance gene to fusarium wilt Foc1 in white cabbage breeding material. At the beginning of the work, 14 molecular markers taken from the VegMarks database and published sources were tested on isogenic white cabbage lines with contrasting resistance to fusarium wilt (resistant line DT-46 and susceptible line Kb1P). InDel marker M10 and SSR markers Frg13 and Ol10-D01 have been ascertained to show polymorphism between white cabbage forms with contrasting resistance to fusarium wilt. Also, PCR analysis of segregating F2 population plants of hybrid combination DT-46 × Kb1P using these markers and phytopathology testing have been conducted. As a result of statistical analysis of segregation, it has been found that only SSR marker Ol10-D01 is co-segregated with the trait of resistance to fusarium wilt as expected segregation of F2 plants by genotype 1 : 2 : 1 according to Mendel’s law; the least recombination frequency between the resistance gene Foc1 and the marker (1.6%) has just been observed only by this locus and it is highly polymorphic (PIC = 0.51).

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Funding

The study was carried out with the financial support of the Kuban Science Foundation and the Federal Scientific Rice Centre within the framework of the scientific project no. MFI-P-20.1/41.

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Authors and Affiliations

  1. Federal Scientific Rice Centre, 350921, Krasnodar, Russia

    E. V. Dubina, Yu. A. Makukha, S. V. Garkusha, O. L. Gorun & S. A. Lesnyak

  2. Trubilin Kuban State Agrarian University, 350044, Krasnodar, Russia

    E. V. Dubina

  3. Federal Research Centre Vavilov All-Russian Institute of Plant Genetic Resources, 190031, St. Petersburg, Russia

    A. M. Artem’eva & D. A. Fateev

Authors
  1. E. V. Dubina
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  2. Yu. A. Makukha
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  3. A. M. Artem’eva
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  4. D. A. Fateev
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  5. S. V. Garkusha
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  6. O. L. Gorun
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  7. S. A. Lesnyak
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Correspondence to E. V. Dubina.

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The authors declare that they have no conflicts of interest.

This article does not contain any studies using animals as an object.

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Dubina, E.V., Makukha, Y.A., Artem’eva, A.M. et al. Molecular Marking in Brassica oleracea L. Breeding for Resistance to Fusarium Wilt. Russ J Genet 59, 1004–1010 (2023). https://doi.org/10.1134/S1022795423100034

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