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Chromosomal localization of aromatic alcohol dehydrogenase fast-migrating isoenzyme Aadh1F (CAD1-F) gene in Triticum aestivum L. bread wheat

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Abstract

Differences in isoenzyme pattern of aromatic alcohol dehydrogenase, NADP-AADH or CAD, were found in the Triticum aestivum L. winter bread wheat cultivars by the method of electrophoresis in the starch gel. A standard three-component spectrum is present in the cv. Zitnica (former Yugoslavia); additional fact-migrating isoenzymes appear in the cv. Novosibirskaya 9 (Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Russia). The presence of fast-migrating CAD isoenzymes is designated as FF phenotype; their absence, as 00 phenotype. Hybridological analysis was carried out; the excess of “null” genotypes was found in F2 progenies. Hybridization with nulli-tetrasomic lines of the chromosomes of the fifth homeologous group was conducted for the gene localization. The segregation analysis demonstrated the most probable localization of the CAD1-F gene in the chromosome 5A. The plants with FF and 00 genotypes differed in a number of chemical and anatomical traits, as well as in grain productivity. The results obtained are discussed in connection with the function of this enzyme in the wheat plant tissues.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    A. A. Konovalov, N. P. Goncharov & E. Ya. Kondratenko

  2. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    I. K. Shundrina & E. V. Karpova

  3. Novosibirsk State University, Novosibirsk, 630090, Russia

    I. K. Shundrina & E. V. Karpova

  4. Department of Selection, Genetics, and Forestry, Novosibirsk State Agrarian University, Novosibirsk, 630039, Russia

    N. P. Goncharov

Authors
  1. A. A. Konovalov
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  2. I. K. Shundrina
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  3. E. V. Karpova
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  4. N. P. Goncharov
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  5. E. Ya. Kondratenko
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Correspondence to A. A. Konovalov.

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Original Russian Text © A.A. Konovalov, I.K. Shundrina, E.V. Karpova, N.P. Goncharov, E.Ya. Kondratenko, 2016, published in Genetika, 2016, Vol. 52, No. 10, pp. 1222–1228.

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Konovalov, A.A., Shundrina, I.K., Karpova, E.V. et al. Chromosomal localization of aromatic alcohol dehydrogenase fast-migrating isoenzyme Aadh1F (CAD1-F) gene in Triticum aestivum L. bread wheat. Russ J Genet 52, 1110–1116 (2016). https://doi.org/10.1134/S1022795416080056

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  • DOI: https://doi.org/10.1134/S1022795416080056

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