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Dynamics of Phospholipid Content in the Vacuolar Membrane of Red Beet Taproots Exposed to Abiotic Stress

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Abstract

The composition of vacuolar membrane phospholipids in the taproot of red beet (Beta vulgaris L.), cv. Modana, was determined at normal conditions and under different types of stress (hypo- and hyperosmotic and oxidative stress). The experiments have shown that, among vacuolar membrane phospholipids in red beet taproot, phosphatidylcholines and phosphatidylethanolamines dominated and accounted for 70% of total phospholipids. It is interesting that the content of phosphatidic acid was high (20% of total phospholipids of the vacuolar membrane). Stress effects brought about changes in the composition of membrane phospholipids, which may be an element of phenotypic adaptation. Under hypoosmotic stress, reliable changes in the content of phosphatidic acid were observed, hyperosmotic stress was associated with changes in the level of phosphatidylcholines and phosphatidylinositols, and oxidative stress was notable for changes in the content of phosphatidylethanolamines and phosphatidylserines. The most significant changes were observed in the classes of phospholipids that may be involved in structural modification of membranes associated with transformation of their bilayer lamellar structure into hexagonal. These phospholipids comprise phosphatidic acid, phosphatidylcholines, and phosphatidylethanolamines. Revealed changes in the content of these phospholipids may alter the ratio between lamellar bilayer and nonbilayer hexagonal lipid structures in the vacuolar membrane and act as an important adaptation mechanism ensuring protection against stress.

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Abbreviations

PA:

phosphatidic acid

PC:

phosphatidylcholines

PE:

phosphatidylethanolamines

PI:

phosphatidylinositols

PL:

phospholipids

PS:

phosphatidylserines

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, Irkutsk, 664033, Russia

    N. V. Ozolina, V. V. Gurina, I. S. Nesterkina & V. N. Nurminskii

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  1. N. V. Ozolina
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  2. V. V. Gurina
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  3. I. S. Nesterkina
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  4. V. N. Nurminskii
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Correspondence to N. V. Ozolina.

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Original Russian Text © N.V. Ozolina, V.V. Gurina, I.S. Nesterkina, V.N. Nurminskii, 2018, published in Fiziologiya Rastenii, 2018, Vol. 65, No. 5, pp. 358–365.

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Ozolina, N.V., Gurina, V.V., Nesterkina, I.S. et al. Dynamics of Phospholipid Content in the Vacuolar Membrane of Red Beet Taproots Exposed to Abiotic Stress. Russ J Plant Physiol 65, 702–708 (2018). https://doi.org/10.1134/S1021443718040088

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  • DOI: https://doi.org/10.1134/S1021443718040088

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