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Influence of phytohormones on morphology and chlorophyll a fluorescence parameters in embryos of Fucus vesiculosus L. (Phaeophyceae)

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Abstract

While a variety of plant hormones from brown algae were described, there were few studies that examined the combined effects of these hormones on morphogenesis and photosynthetic physiology in develo** fucoid embryos. We evaluated the effects of phytohormones to determine the extent, to which responses were similar to those of terrestrial plants. Kinetin, IAA, ABA, GA3, and kinetin + IAA were added to seawater at a physiological concentration (1 mg/L), and embryos of Fucus vesiculosus L. were grown for 10 days. Photosynthetic activity of single embryos or embryo cells were characterized using the following fluorescence parameters: minimum fluorescence yield (F 0), maximum quantum yield (F v/F m), relative maximum rate of electron transfer to photosystem II under saturation irradiances (rETRmax), photosynthetic efficiency under non-saturating irradiances (αETR) and saturation irradiance (E k). In addition, embryo length and diameter and apical hair length and number were determined. Morphological changes associated with hormone treatments included an increase in the embryo length in the presence of IAA, an increase in the embryo diameter in the presence of IAA, kinetin, and kinetin + IAA, an increase in the maximum hair length and number in the presence of kinetin + IAA, and a decrease in the hair length and number in the presence of ABA. With respect to fluorescence parameters, significant effects of phytohormones included an increase in the F 0 and F v/F m at kinetin treatment, a synergistic effect of kinetin + IAA on F v/F m, rETRmax, and αETR, a promotion of F v/F m by GA, and a decrease of the parameters by ABA. These results are consistent with the data on responses of land plants to the same hormones and suggest that brown algae have evolved regulatory mechanisms for morphogenesis and photosynthetic regulation similar to plants.

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Abbreviations

AF:

after fertilization

αETR:

photosynthetic efficiency under non-saturating irradiances

E k :

saturation irradiance

F 0 :

minimum fluorescence yield of PSII photochemistry

F v/F m :

maximum quantum yield of PSII photochemistry

PAM:

pulse amplitude modulation

PS:

photosystem

rETRmax :

relative maximum rate of electron transfer to PSII under saturation irradiances

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Authors and Affiliations

  1. Department of Plant Physiology and Biochemistry, St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia

    E. R. Tarakhovskaya

  2. Department of Oceanography, Chonnam National University, Gwangju, 500-757, Korea

    E. J. Kang & K. Y. Kim

  3. Department of Biology, St. Francis Xavier University, Antigonish, Nova Scotia, Canada, B2G 2W5

    D. J. Garbary

Authors
  1. E. R. Tarakhovskaya
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  2. E. J. Kang
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  3. K. Y. Kim
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  4. D. J. Garbary
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Correspondence to E. R. Tarakhovskaya.

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Tarakhovskaya, E.R., Kang, E.J., Kim, K.Y. et al. Influence of phytohormones on morphology and chlorophyll a fluorescence parameters in embryos of Fucus vesiculosus L. (Phaeophyceae). Russ J Plant Physiol 60, 176–183 (2013). https://doi.org/10.1134/S1021443713020192

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  • DOI: https://doi.org/10.1134/S1021443713020192

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