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Biocompatible Molecularly Imprinted Cryogel Matrices for Protein Recognition in Biological Samples

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Abstract

A series of molecularly imprinted and nonimprinted cryogels based on the copolymer of 2‑hydroxyethyl methacrylate and N,N '-methylene-bis(acrylamide) are synthesized at various ratios and contents of comonomers in the polymerization mixture. The resulting materials are characterized in terms of surface morphology, swelling ability, average pore size, porosity, and compression mechanical characteristics. In addition, a comparative assessment of the adsorption properties of the molecularly imprinted and nonimprinted cryogels with respect to a target protein (α-chymotrypsin) in the dynamic mode is performed, as well as the in vitro biocompatibility of the cryogels using two cell lines.

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ACKNOWLEDGMENTS

This work was carried out using the equipment of recourse centers for X-Ray Diffraction Studies and Geo-Environmental Research and Modeling (GEOMODEL) at the St. Petersburg State University Research Park.

Funding

This work was carried out within the scope of megagrant of the Ministry of Science and Higher Education of the Russian Federation (no. 075-15-2021-637).

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Authors and Affiliations

  1. Institute of Chemistry, St. Petersburg State University, 198504, St. Petersburg, Russia

    M. S. Leonovich, V. A. Korzhikov-Vlakh, I. A. Gur’yanov, O. N. Brevnov & T. B. Tennikova

  2. Institute of Macromolecular Compounds, Russian Academy of Sciences, 199004, St. Petersburg, Russia

    M. S. Leonovich & E. G. Korzhikova-Vlakh

Authors
  1. M. S. Leonovich
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  2. V. A. Korzhikov-Vlakh
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  3. E. G. Korzhikova-Vlakh
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  4. I. A. Gur’yanov
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  5. O. N. Brevnov
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  6. T. B. Tennikova
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Correspondence to V. A. Korzhikov-Vlakh.

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Translated by T. Soboleva

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Leonovich, M.S., Korzhikov-Vlakh, V.A., Korzhikova-Vlakh, E.G. et al. Biocompatible Molecularly Imprinted Cryogel Matrices for Protein Recognition in Biological Samples. Polym. Sci. Ser. A (2024). https://doi.org/10.1134/S0965545X24600479

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  • DOI: https://doi.org/10.1134/S0965545X24600479

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