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Specific Features of Formation of Multispecies Microbial Biofilms on Polyethylene Surface

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Abstract

Patterns of formation of three-species biofilms on the surface of an LDPE-based composite material were studied using a mixture of pure planktonic cultures of microorganisms capable of degrading this polymer. This is the first report of the role of the ascomycete Yarrowia lipolytica as a primary colonizer in this system, forming aggregates with gram-negative bacteria Pseudomonas aeruginosa or Chromobacterium violaceum. Addition of biocidal compounds of the guanidine family to LDPE as a component of the filler was shown to result in selective suppression of biofilm growth of some microorganisms, while it had no effect on the initial stages of adhesion and on planktonic growth of the cultures. Gram-positive bacteria of the genus Kocuria exhibited the highest sensitivity to these biocidal agents.

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REFERENCES

  1. Ashmarin, I.P. and Vorob’ev, A.A., Statisticheskie metody v mikrobiologicheskikh issledovaniyakh (Statistical Methods in Microbiological Research), Leningrad: Izd. Med. Lit., 1962.

  2. Coenye, T. and Nelis, H.J., In vitro and in vivo model systems to study microbial biofilm formation, J. Microbiol. Methods, 2010, vol. 83, pp. 89–105.

    Article  CAS  Google Scholar 

  3. Gerasin, V.A., Sivov, N.A., Menyashev, M.R., Mendeleev, D.I., Yakovleva, A.V., and Serdyukov, D.V., RF Patent no. 2679147, Byull. Izobret., 2019, no. 4.

  4. Hassanpourfard, M., Sun, X., Valiei, A., Mukherjee, P.,Thundat, T., Liu, Y., and Kumar, A., Protocol for biofilm streamer formation in a microfluidic device with micro-pillars, J. Vis. Exp., 2014, vol. 90. https://doi.org/10.3791/51732

  5. Kallistova, A.Yu., Sabrekov, A.F., Goncharov, V.M., Pimenov, N.V., and Glagolev, M.V., On the application of statistical analysis for interpretation of experimental results in environmental microbiology, Microbiology (Moscow), 2019, vol. 88, pp. 232–239.

    Article  CAS  Google Scholar 

  6. Khar’kova, E.M., Mendeleev, D.I., Guseva, M.A., and Gerasin, V.A., Structure and properties of polymer-polymer composites based on biopolymers and ultra-high molecular weight polyethylene obtained via ethylene in situ polymerization, J. Polym. Environ., 2019, vol. 27, pp. 165−175.https://doi.org/10.1007/s10924-018-1326-0

  7. Pitts, B., Hamilton, M.A., Zelver, N., and Stewart, P.S., A microtiter-plate screening method for biofilm disinfection and removal, J. Microbiol. Methods, 2003, vol. 54, pp. 269–276.

    Article  CAS  Google Scholar 

  8. Plakunov, V.K., Mart’yanov, S.V., Teteneva, N.A., and Zhurina, M.V., A universal method for quantitative characterization of growth and metabolic activity of microbial biofilms in static models, Microbiology (Moscow), 2016, vol. 85, pp. 509–513.

    Article  CAS  Google Scholar 

  9. Raddadi, N. and Fava, F., Biodegradation of oil-based plastics in the environment: existing knowledge and needs of research and innovation, Sci. Total Environ., 2019, vol. 679, pp. 148‒158.

    Article  CAS  Google Scholar 

  10. Shatalov, D.O., Kedik, S.A., Zhavoronok, E.S., Aydakova, A.V., Ivanov, I.S., Evseeva, A.S., Beliakov, S.V., Biryulin, S.I., Kovalenko, A.V., and Mikhailenko, E.N., The current state and development of perspectives of application of synthetic antimicrobial agents, Polymer Sci. Ser. D, 2017, vol. 10, pp. 293–299.

    Article  CAS  Google Scholar 

  11. Strelkova, E.A., Zhurina, M.V., Plakunov, V.K., and Belyaev, S.S., Stimulation of biofilm formation by antibiotics, Microbiology (Moscow), 2012, vol. 81, pp. 259–262.

    Article  CAS  Google Scholar 

  12. Vandecandelaere, I., Van Acker, H., and Coenye, T., A microplate-based system as in vitro model of biofilm growth and quantification, Methods Mol. Biol., 2016, vol. 1333, pp. 53‒66.

    Article  CAS  Google Scholar 

  13. Yoon, M.G., Jeon, H.J., and Kim, M.N., Biodegradation of polyethylene by a soil bacterium and AlkB cloned recombinant cell, J. Bioremed. Biodegrad., 2012, vol. 3, article 145. https://doi.org/10.4172/2155-6199.1000145

    Article  CAS  Google Scholar 

  14. Zhurina, M.V., Gannesen, A.V., Martyanov, S.V., and Plakunov, V.K., Express method for determining the relation between polyethylene biocorrosion by Chromobacterium violaceum biofilms and their ability to form extracellular matrix, Microbiology (Moscow), 2020, vol. 89, pp. 44‒49.

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Russian Foundation for Basic Research, project no. mk-18-29-05048 and partially supported by the Ministry of Science and Higher Education of the Russian Federation.

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Authors and Affiliations

  1. Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    M. V. Zhurina, A. Yu. Kallistova, A. E. Panyushkina, A. V. Gannesen, S. V. Mart’yanov & V. K. Plakunov

  2. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, 119991, Moscow, Russia

    V. A. Gerasin, N. A. Sivov & V. A. Tikhomirov

Authors
  1. M. V. Zhurina
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  2. A. Yu. Kallistova
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  3. A. E. Panyushkina
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  4. A. V. Gannesen
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  5. S. V. Mart’yanov
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  6. V. A. Gerasin
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  7. N. A. Sivov
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  8. V. A. Tikhomirov
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  9. V. K. Plakunov
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Correspondence to M. V. Zhurina.

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The authors declare no conflict of interest. This article does not contain any studies involving animals or human participants performed by the authors.

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Translated by A. Panyushkina

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Zhurina, M.V., Kallistova, A.Y., Panyushkina, A.E. et al. Specific Features of Formation of Multispecies Microbial Biofilms on Polyethylene Surface. Microbiology 89, 396–404 (2020). https://doi.org/10.1134/S0026261720040165

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  • DOI: https://doi.org/10.1134/S0026261720040165

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