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Phosphonoacetate biosynthesis: In vitro detection of a novel NADP+-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter

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Abstract

A novel phosphonoacetaldehyde-oxidizing activity was detected in cell-extracts of the marine bacterium Roseovarius nubinhibens ISM grown on 2-aminoethylphosphonic acid (2-AEP; ciliatine). Extracts also contained 2-AEP transaminase and phosphonoacetate hydrolase activities. These findings indicate the existence of a biological route from 2-AEP via phosphonoacetaldehyde for the production of phosphonoacetate, which has not previously been shown to be a natural product. The three enzymes appear to constitute a previously-unreported pathway for the mineralization of 2-AEP which is a potentially important source of phosphorus in the nutrient-stressed marine environment.

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Authors and Affiliations

  1. School of Biological Science, Queens University, Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, Northern Ireland, UK

    N. A. Cooley, A. N. Kulakova, J. F. Villarreal-Chiu, J. W. McGrath & J. P. Quinn

  2. Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL, 60439, USA

    J. A. Gilbert

  3. Department of Ecology and Evolution, University of Chicago, 5640 South Ellis Avenue, Chicago, IL, 60637, USA

    J. A. Gilbert

Authors
  1. N. A. Cooley
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  2. A. N. Kulakova
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  3. J. F. Villarreal-Chiu
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  4. J. A. Gilbert
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  5. J. W. McGrath
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  6. J. P. Quinn
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Correspondence to J. W. McGrath.

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Cooley, N.A., Kulakova, A.N., Villarreal-Chiu, J.F. et al. Phosphonoacetate biosynthesis: In vitro detection of a novel NADP+-dependent phosphonoacetaldehyde-oxidizing activity in cell-extracts of a marine Roseobacter . Microbiology 80, 335–340 (2011). https://doi.org/10.1134/S0026261711030076

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