Abstract
The effect of solute hydrogen bonding capacity on the osmotic stability of lysosomes was examined through measurement of free enzyme activity of lysosomes after their incubation in sucrose and poly(ethylene glycol) (PEG) (1500–6000 Da molecular mass) media. Free enzyme activity of the lysosomes was less in the PEG medium than that in the sucrose medium under the same hypotonic condition. The lysosomal enzyme latency loss decreased with increasing hydrogen bonding capacity of the solute. In addition, the lysosomes lost less latency at lower incubation temperature. The results indicate that solute hydrogen bonding capacity plays an important role in the osmotic protection of an incubation medium to lysosomes.
Similar content being viewed by others
Abbreviations
- PEG:
-
poly(ethylene glycol)
References
Lloyd, J. B., and Forster, S. (1986) Trends Biol. Sci., 11, 365–368.
Iveson, G. P., Bird, S. J., and Lloyd, J. B. (1989) Biochem. J., 261, 451–456.
Lloyd, J. B. (1992) in Pathophysiology of Lysosomal Transport (Thoene, J. G., ed.) Chap. 7, CRC Press, Boca Raton, pp. 295–308.
Jonas, A. J., Smith, M. L., Allison, W. S., Laikind, P. K., Greene, A. A., and Schneider, J. A. (1983) J. Biol. Chem., 258, 11727–11730.
Yamada, H., Hayashi, H., and Natori, Y. (1984) J. Biochem., 95, 1155–1160.
Greene, A. A., and Schneider, J. S. (1992) in Pathophysiology of Lysosomal Transport (Thoene, J. G., ed.) Chap. 2, CRC Press, Boca Raton, pp. 7–44.
Bird, S. J., Forster, S., and Lloyd, J. B. (1987) Biochem. J., 245, 929–931.
Diamond, J. M., and Wright, E. M. (1969) Annu. Rev. Physiol., 31, 581–646.
Ross, P. D., and Rekharsky, M. V. (1996) Biophys. J., 71, 2144–2154.
Rowlinson, J. S. (1957) in Hydrogen Bonding (Hadzi, D., ed.) Pergamon Press, London, pp. 423–427.
Hammel, H. T., and Schlegel, W. M. (2005) Cell Biochem. Biophys., 42, 277–345.
Zhao, Z. J., Wang, Q., Zhang, L., and Wu, T. (2008) J. Phys. Chem. B, 112, 7515–7521.
Eggena, P. (1983) Am. J. Physiol., 244, C44–9.
Avdeef, A., and Tsinman, O. (2006) Eur. J. Pharm. Sci., 28, 43–50.
Orme, F. W., Moronne, M. M., and Macey, R. I. (1988) J. Membr. Biol., 104, 57–68.
Deamer, D. W., and Bramhall, J. (1986) Chem. Phys. Lipids, 40, 167–188.
Sharma, M., Resta, R., and Car, R. (2007) Rev. Lett., 98, 247401.
Yoshii, N., Miura, S., and Okazaki, S. (2001) Lett., 345, 195–200.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in Russian in Biokhimiya, 2009, Vol. 74, No. 12, pp. 1672–1677.
Rights and permissions
About this article
Cite this article
Hao, SJ., Hou, JF. & Zhang, GJ. Effect of solute hydrogen bonding capacity on osmotic stability of lysosomes. Biochemistry Moscow 74, 1363–1367 (2009). https://doi.org/10.1134/S0006297909120104
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0006297909120104