Extended Data Fig. 7: Isoforms with extended 3′UTR do not produce proteins.
From: Alternative polyadenylation by sequential activation of distal and proximal PolyA sites
a, Illustration of alternative UTR (aUTR) and its insertion in the 3′UTR of GFP reporter gene. b, The effect of aUTR from CPSF6 and FUBP1 on the expression reporter detected by WB. Another flag expression plasmid was used as transfection control. Data were from n = 2 independent experiments. c, Two models for the processing of the proximal polyA sites. Model 1: the proximal and distal polyA sites are processed independently from each other (left); Model 2: the processing of distal polyA site is prior to the proximal one and the dPAS isoform acts as the intermediate for the proximal one (right). d, Validation of the inserted ribozyme by Sanger sequencing.