Extended Data Fig. 9: Impaired microglia dynamics in Mg^PTX mice.

a, In vivo 2 P time-lapse imaging of individual microglial process extensions and retractions in awake Mg^WT and Mg^PTX mice during whisker stimulation (‘Stim’) and non-stimulation (‘Unstim’) conditions. Data are mean ± s.e.m. n = 6 mice per genotype. ^*P = 0.018 (Mg^WT Stimulated vs. all other conditions) and ^***P = 0.0083 (Mg^WT vs. Mg^PTX mice) by two-way ANOVA. b, Velocity (blue vectors) of individual microglia process dynamics (white) in Mg^WT and Mg^PTX mice. Scale bar, 25 µm. Data are mean ± s.e.m. n = 6 mice per genotype. No significant difference by two-way ANOVA with post hoc Tukey’s multiple comparison test. c, Ca²⁺ peak amplitudes of whisker stimulation-induced neuronal Ca²⁺ transients in Mg^PTX and Mg^WT mice. Data are mean ± s.e.m. n = 14 Mg^WT and n = 9 Mg^PTX mice. No significant difference bytwo-sample t-test of mean AUC and Permutation test.

Source data