Extended Data Fig. 3: Molecular signatures of HSPCs replenished from single Vwf-tdTomato⁻ Multi-HSCs or Vwf-tdTomato⁺ P-HSCs.

a, UMAP plots (as in Fig. 3b) of HSPC cell surface antigens; mRNA expression (left) and protein/reporter expression based on index sorting information from the same cells (right). b, UMAP plots of single LIN⁻cKIT⁺ HSPCs from mice reconstituted by single Vwf-tdTomato⁻ Multi-HSCs or Vwf-tdTomato⁺ P-HSCs. Color scales indicate expression of molecular signatures (AUCell scores) for HSCs (MolO), lineage restriction (MkP, erythroid, myeloid, and lymphoid), and cell cycle (G2M and S phases).