Extended Data Fig. 10: Mitochondria transfer into CD4+ T cells protects synovial tissue from inflammation.
From: Mitochondrial aspartate regulates TNF biogenesis and autoimmune tissue inflammation
Mitochondria were isolated from healthy T cells and transferred into RA CD4+ T cells prior to their adoptive transfer into synovium-NSG chimeras. Explanted synovial grafts were analyzed by immunohistochemical staining and tissue transcriptomics (RT-PCR). 8 tissues in each group. (a) H&E staining of synovial tissue sections. Scale bar; 50 μm. (b) Immunofluorescence staining for CD3+ T cells in synovial infiltrates. Representative images. Scale bar; 10 μm. (c) Gene expression profiling (RT-PCR) of TRB, TBET, RORG and other key inflammatory markers (n = 8). All data are mean ± SEM. Two-tailed unpaired Mann-Whitney-Wilcoxon rank test. *P < 0.05, **P < 0.01, ***P < 0.001.