In type I-F CRISPR–Cas systems, Cas2/3 is typically recruited to the interference complex Cascade by Cas8f. We resolved the structures of the phage ICP1 CRISPR–Cas complexes and discovered a Cas2/3 recruitment mechanism distinct from that in other type I-F systems — recruitment by Cas1.
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References
Makarova, K. S. et al. Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants. Nat. Rev. Microbiol. 18, 67–83 (2019). A review article that provides an overview of CRISPR–Cas systems.
Rollins, M. F. et al. Structure reveals a mechanism of CRISPR-RNA-guided nuclease recruitment and anti-CRISPR viral mimicry. Mol. Cell 74, 132–142 (2019). This paper reports that Cas8f recruits Cas2/3 in type I-F systems.
Seed, K. D., Lazinski, D. W., Calderwood, S. B. & Camilli, A. A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity. Nature 494, 489–491 (2013). This paper is an early report of a phage-encoded CRISPR–Cas system.
Sternberg, S. H., Richter, H., Charpentier, E. & Qimron, U. Adaptation in CRISPR-Cas systems. Mol. Cell 61, 797–808 (2016). A review article that provides an overview of adaptation in CRISPR–Cas systems.
Staals, R. H. et al. Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system. Nat. Commun. 7, 12853 (2016). This paper reports the interference-driven spacer acquisition in type I-F systems.
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This is a summary of: Zhang, L. et al. Cas1 mediates the interference stage in a phage-encoded CRISPR–Cas system. Nat. Chem. Biol. https://doi.org/10.1038/s41589-024-01659-5 (2024).
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An alternative mechanism for recruiting Cas2/3 in a phage-encoded CRISPR–Cas system. Nat Chem Biol (2024). https://doi.org/10.1038/s41589-024-01667-5
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DOI: https://doi.org/10.1038/s41589-024-01667-5
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