Extended Data Fig. 6: Visualization of cRGD/c-Met AMR system upon sensing and transmitting integrin-mediated intercellular force on Hela cells.
From: DNA-functionalized artificial mechanoreceptor for de novo force-responsive signaling
a, Representative images of brightfield, Cy5, FAM and TAMRA channels for the Hela cells treated with no cRGD conjugated AMR (nRGD/c-Met AMR) or cRGD/c-Met AMR (400 nM). For the actomyosin inhibition, Hela cells were pre-treated with Blebbistatin (10 µM) for 10 min before the addition of cRGD/c-Met AMR. Scale bar, 10 µm. b, Quantification of the relative FAM/Cy5 and TAMRA/Cy5 ratio to characterize the F-TSDR at cell-cell junctions. Data represent mean ± S.D. from independent experiments (n = 10). Significance was determined by unpaired two-tailed Student’s t-test.