Supplementary Figure 3: Chromatin accessibility and DNA methylation level around the center of NDRs and NORs.
From: Single-cell multi-omics sequencing of human early embryos
(a) Diagram of the number of TSS NDRs (NDRs containing TSS), proximal NDRs (NDRs within 2 kb upstream and downstream of the TSS) and distal NDRs (NDRs at least 2 kb away from the TSS) called at each stage from pools of samples. The last row named ‘Total’ is the total number of NDRs called from oocytes to hESCs. Overlap** NDRs longer than 10 bp were elongated and combined as one NDR. (b) Boxplot showing the dynamics of the length of TSS NDR distributions during human preimplantation development. The number of TSS NDR in each stage are shown in Supplementary Fig. 3a. Each box represents median and 25% and 75% quantiles, while whiskers indicate 1.5 times of IQR. (c) Chromatin accessibility and DNA methylation levels around the center of proximal NDRs and distal NDRs. (d) Chromatin accessibility and DNA methylation levels around the center of NORs. The sample size in a-d corresponds to the euploid sample sizes provided in Fig. 1a (expect for 8-cell, n = 31 cells).