Supplementary Figure 2: Chromatin accessibility around transcription start sites.
From: Single-cell multi-omics sequencing of human early embryos
(a) Chromatin accessibility 2 kb upstream of the TSS, within the gene body, and 2 kb downstream of the TES of pools of samples across each stage. (b) Chromatin accessibility (upper panel) and DNA methylation level (lower panel) of H3K4me3 marked (in red) and unmarked (in blue) promoters. (c) Chromatin accessibility and DNA methylation levels of promoters with different expression levels. Genes were divided into 4 groups, including highly expressed (RPKM > 10), intermediately expressed (1 < RPKM ≤ 10), and lowly expressed genes (0.1 < RPKM ≤ 1) and genes that were not expressed (RPKM ≤ 0.1). The sample size in b-c corresponds to 3 euploid hESCs. (d) GO analysis of 8,385 genes that turned into an open state after fertilization (Related to Fig. 1d). The sample size in a, d corresponds to the euploid sample sizes provided in Fig. 1a (expect for 8-cell, n = 31 cells).