Extended Data Figure 5: Related to Fig. 3.
From: MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5′ end resection
a, Telomeric single-stranded G-overhang assay of TRF2ts MEFs transduced with control or Mad2l2 sh4 shRNAs, showing that the increase in overhang signal upon Mad2l2 knockdown is due to 3′ terminal sequences because the signal is removed by treatment with Escherichia coli 3′ exonuclease Exo1. b, Mad2l2 knockdown causes increased single-stranded telomeric G-overhang signals in TRF2ts Lig4−/− MEFs. c, Quantification of relative telomeric G-overhang signals in TRF2ts Lig4−/− MEFs transduced with control or Mad2l2 shRNAs and grown at 32 °C or for 12 or 24 h at 39 °C (n = 2, mean ± s.e.m.). d, qRT–PCR analysis of Mad2l2 expression levels in TRF2ts Lig4−/− MEFs infected with control or Mad2l2 shRNA lentivirus (error bars denote s.d.). e, Survival assays of TRF2ts MEFs transduced with control or Mad2l2 shRNAs and subsequently with control, Ctip or Exo1 shRNAs. f, Quantification of the survival assays shown in e. g, qRT–PCR analysis of Ctip and Exo1 expression levels of cells shown in e, f and h and in Fig. 3f (error bars denote s.d.). h, Growth curves at 39 °C of TRF2ts MEFs transduced with non-targeting control or Mad2l2 shRNAs and subsequently with control, Ctip or Exo1 shRNAs (error bars denote s.e.m.).