Log in

Micropreparative LC of proteins

  • Product Review
  • Published:

From Nature

View current issue Submit your manuscript

The use of short microbore HPLC columns with different selectivity for the purification of proteins and peptides at the picomole level is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Esch, F. S. Analyt. Biochem. 136, 39–47 (1984).

    Article  CAS  Google Scholar 

  2. Hunkapiller, M.W. & Hood, L.E. Science 219, 650–659 (1983).

    Article  ADS  CAS  Google Scholar 

  3. Kucera, P. J. Chromatogr. 198, 93 (1980).

    Article  CAS  Google Scholar 

  4. Nice, E.C., Lloyd, C.J. & Burgess, A.W. J. Chromatogr. 296, 153–170 (1984).

    Article  CAS  Google Scholar 

  5. Moore, R.M. & Walters, R.R. J. Chromatogr. 317, 119–128 (1984).

    Article  CAS  Google Scholar 

  6. Nice, E.C., Grego, B. & Simpson, R. Biochem. Int. 11, 187–195 (1985).

    CAS  Google Scholar 

  7. Simpson, R.J., Moritz, R.L., Begg, G.S., Rubira, M.R. & Nice, E.C. Analyt. Biochem. 177, 221–236 (1989).

    Article  CAS  Google Scholar 

  8. Simpson, R.J., Moritz, R.L., Nice, E.C. & Grego, B. Eur. J. Biochem. 165, 21–29 (1987).

    Article  CAS  Google Scholar 

  9. Simpson, R.J. & Moritz, R.L. J. Chromatogr. 474, 418–423 (1989).

    Article  CAS  Google Scholar 

  10. Rubenstein, M. Analyt. Biochem. 98, 1–7 (1979).

    Article  Google Scholar 

  11. Bij, K.E., Horváth, Cs., Melander, W.R. & Naham, A. J. Chromatogr. 203, 65–84 (1985).

    Article  Google Scholar 

  12. Hearn, M.T.W. & Grego, B. J. Chromatogr. 255, 125–136 (1981).

    Article  Google Scholar 

  13. Ward, L.D., Reid, G.E., Moritz, R.L. & Simpson, R.J. in Current Research in Protein Chemistry (ed. Villafrance, J.J.) (Academic, New York, 1990).

    Google Scholar 

  14. Nice, E.C. & Simpson, R.J. J. Pharmaceut. Biomed. Anal. 7, 1039–1053 (1989).

    Article  CAS  Google Scholar 

  15. Simpson, R.J. & Nice, E.C. in The Use of HPLC in Receptor Biochemistry (ed. Kerlavage, A.R.) 201–244 (A.R. Liss, New York, 1989).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nice, E. Micropreparative LC of proteins. Nature 348, 462–463 (1990). https://doi.org/10.1038/348462a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/348462a0

  • Springer Nature Limited

This article is cited by

Navigation