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Effect of Double-Stranded RNA on the Expression of Epithelial Neutrophil Activating Peptide-78/CXCL-5 in Human Endothelial Cells

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Abstract

Epithelial neutophil activating peptide-78 (ENA-78)/CXCL-5 is a member of CXC chemokines. ENA-78 was originally described as a factor produced by epithelial cells only. But other types of cells including vascular endothelial cells also produce it. ENA-78 production by endothelial cells may be important for the regulation of neutrophil activation in inflammatory reactions. Polyinosinic-polycytidylic acid (poly IC) is a synthetic double-stranded RNA, which mimics the viral infection when applied to cells and affects the expression of various genes related to inflammatory reactions. In the present study, we examined the effect of poly IC on the expression of ENA-78 in human umbilical vein endothelial cells (HUVEC). HUVEC in culture were treated with poly IC and the expression of ENA-78 mRNA and protein were analyzed by reverse-transcription polymerase chain reaction and enzyme-linked immunosorbent assay. Poly IC induced ENA-78 expression in time- and concentration-dependent manners. Th2-type cytokine IL-4 partially inhibited the induction of ENA-78 by poly IC. 2-Aminopurine, an inhibitor of dsRNA-dependent kinase, suppressed the induction of ENA-78 by poly IC. ENA-78 may be involved in the inflammatory reactions elicited by viral infection in endothelial cells.

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REFERENCES

  1. Walz, A., R. Burgener, B. Car, M. Baggiolini, S. L. Kunkel, and P. M. Strieter. 1991. Structure and neutrophil-activating properties of a novel inflammatory peptide (ENA-78) with homology to inter-leukin 8. J. Exp. Med. 174:1355–1362.

    Google Scholar 

  2. Schnyder-Candrian, S., and A. Walz. 1997. Neutrophil-activating protein ENA-78 and IL-8 exhibit different patterns of expression in lipopolysaccharide-and cytokine-stimulated human monocytes. J. Immunol. 158:3888–3894.

    Google Scholar 

  3. Keates, S., A. C. Keates, E. Mizoguchi, A. Bhan, and C. P. Kelly. 1997. Enterocytes are the primary source of the chemokine ENA-78 in normal colon and ulcerative colitis. Am.J.Physiol. 273:G75–G82.

    Google Scholar 

  4. Imaizumi, T., K. H. Albertine, D. L. Jicha, T. M. McIntyre, S. M. Prescott, and G. A. Zimmerman. 1997. Human endothelial cells synthesize ENA-78: relationship to IL-8 and to signaling of PMN adhesion. Am. J. Respir. Cell Mol. Biol. 17:181–192.

    Google Scholar 

  5. Nasu, K., K. Arima, K. Kai, K. Fujisawa, M. Nishida, and I. Miyakawa. 2001. Expression of epithelial neutrophil-activating peptide 78 in cultured human endometrial stromal cells. Mol. Hum. Reprod. 7:453–458.

    Google Scholar 

  6. Suzuki, S., M. Kobayashi, K. Chiba, I. Horiuchi, J. Wang, T. Kondoh, S. Hashino, J. Tanaka, M. Hosokawa, and M. Asaka. 2002. Autocrine production of epithelial cell-derived neutrophil attractant-78 induced by granulocyte colony-stimulating factor in neutrophils. Blood 99:1863–1865.

    Google Scholar 

  7. Fillmore, R. A., S. E. Nelson, R. N. Lausch, J. E. Oakes. 2003. Differential regulation of ENA-78 and GCP-2 gene expression in human corneal keratocytes and epithelial cells. Invest. Ophthalmol. Vis. Sci. 44:3432–3437.

    Google Scholar 

  8. Bozic, C. R., N. P. Gerard, and C. Gerard. 1996. Receptor binding specificity and pulmonary gene expression of the neutrophil-activating peptide ENA-78. Am. J. Resp. Cell Mol. 14:302–308.

    Google Scholar 

  9. Z'Graggen, K., A. Walz, L. Mazzucchelli, R. M. Strieter, and C. Mueller. 1997. The C-X-C chemokine ENA-78 is preferentially expressed in intestinal epithelium in inflammatory bowel disease. Gastroenterology 113:808–816.

    Google Scholar 

  10. Rieder, G., W. Einsiedl, R. A. Hatz, M. Stolte, G. A. Enders, and A. Walz. 2001. Comparison of CXC chemokines ENA-78 and interleukin-8 expression in Helicobacter pylori-associated gastritis. Infect. Immun. 69:81–88.

    Google Scholar 

  11. Koch, A. E., M. V. Volin, J. M. Woods, S. L. Kunkel, M. A. Connors, L. A. Harlow, D. C. Woodruff, M. D. Burdick, and R. M. Strieter. 2001. Regulation of angiogenesis by the C-X-C chemokines interleukin-8 and epithelial neutrophil activating peptide 78 in the rheumatoid joint. Arthritis Rheum. 44:31–40.

    Google Scholar 

  12. Donninger, H., R. Glashoff, H. M. Haitchi, J. A. Syce, R. Ghildyal, E. van Rensburg, and P. G. Bardin. 2003. Rhinovirus induction of the CXC chemokine epithelial neutrophil activating peptide 78 in bronchial epithelium. J. Infect. Dis. 187:1809–1817.

    Google Scholar 

  13. Imaizumi, T., M. Kumagaia, M. Hatakeyama, W. Tamo, K. Yamashita, H. Yoshida, H. Munakata, and K. Satoh. 2003. Effect of 15-deoxy-Δ12 14-prostaglandin J2 on IL-1-induced expression of epithelial neutrophil-activating protein-78 in human endothelial cells. Prostaglandins Leukot. Essent. Fatty Acids. 69: 323–327.

    Google Scholar 

  14. Offermann, M. K., J. Zimring, K. H. Mellits, M. K. Hagan, R. Shaw, R. M. Medford, M. B. Mathews, S. Goodbourn, and R. Jagus. 1995. Activation of the double-stranded-RNA-activated protein kinase and induction of vascular cell adhesion molecule-1 by poly(I).poly(C) in endothelial cells. Eur. J. Biochem. 232:28–36.

    Google Scholar 

  15. Williams, B. R. 1999. PKR; a sentinel kinase for cellular stress. Oncogene 18:6112–6120.

    Google Scholar 

  16. Gale, M. Jr., and M. G. Katze. 1998. Molecular mechanisms of interferon resistance mediated by viral-directed inhibition of PKR, the interferon-induced protein kinase. Pharmacol. Ther. 78:29–46.

    Google Scholar 

  17. Zimmerman, G. A., R. E. Whatley, T. M. McIntyre, D. E. Benson, and S. M. Prescott. 1990. Endothelial cells for studies of platelet-activating factor and arachidonate metabolites. Methods Enzymol. 187:520–535.

    Google Scholar 

  18. Uetani, K., A. D. Der, M. Zamanian-Daryoush, C. Motte, B. Y. Lieberman, B. R. G. Williams, and S. C. Erzurum. 2000. Central role of double-stranded RNA-activated protein kinase in microbial inducetion of nitric oxide synthase. J. Immunol. 265: 988–996.

    Google Scholar 

  19. Gern, J. E., D. A. French, K. A. Grindle, R. A. Brockman-Schneider, S. Konno, and W. W. Busse. 2003. Double-stranded RNA induces the synthesis of specific chemokines by bronchial epithelial cells. Am. J. Resp. Mol. Cell. Biol. 28:731–737.

    Google Scholar 

  20. Heimeier, M. R., A. L. Scarim, and J. A. Corbett. 1998. Double-stranded RNA-induced inducible nitric-oxide synthase expression and interleukin-1 release by murine macrophages requires NF-Κ B activation. J. Biol. Chem. 273:15301–15307.

    Google Scholar 

  21. Steer, S. A., J. M. Moran, L. B. Maggi, R. M. L. Buller, H. Perlman, and J. A. Corbett. 2003. Regulation of cyclooxygenase-2 expres-sion by macrophages in response to double-stranded RNA and viral infection. J. Immunol. 170:1070–1076.

    Google Scholar 

  22. Zimmerman, G. A., T. M. McIntyre, and S. M. Prescott. 1996. Adhesion and signaling in vascular cell-cell interactions. J. Clin. Invest. 98:1699–1702.

    Google Scholar 

  23. Harcourt, J. L., M. K. Hagan, and M. K. Offerman. 2000. Moduration of double-stranded RNA-mediated gene induction by interferon in human umbilical vein endothelial cells. J. Interferon Cytokine Res. 20:1007–1013.

    Google Scholar 

  24. Ishikawa, A., T. Imaiuzmi, H. Yoshida, N. Nishi, T. Nakamura, M. Hirashima, and K. Satoh. 2004. Double-stranded RNA enhances the expression of galectin-9 in vascular endothelial cells. Immunol. Cell Biol. in press.

  25. Kohoe, K. E., M. A. Brown, and F. Imani. 2001. Double-stranded RNA regulates IL-4 expression. J. Immunol. 167: 2496–2501.

    Google Scholar 

  26. Kumar, A., J. Haque, J. Lacoste, J. Hiscott, and B. R. Williams. 1994. Double-stranded RNA-dependent protein kinase activates transcription factor NF-Κ B by phosphorylating IΚ B. Proc. Natl. Acad. Sci. USA 91:6288–6292.

    Google Scholar 

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Authors and Affiliations

  1. Department of Vascular Biology, Institute of Brain Science, Hirosaki University School of Medicine, Hirosaki, Japan

    Tadaatsu Imaizumi, Masaharu Hatakeyama, Akira Ishikawa, Koji Yamashita, Hidemi Yoshida & Kei Satoh

  2. The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan

    Kageaki Taima

Authors
  1. Tadaatsu Imaizumi
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  2. Masaharu Hatakeyama
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  3. Kageaki Taima
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  4. Akira Ishikawa
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  5. Koji Yamashita
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  7. Kei Satoh
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Imaizumi, T., Hatakeyama, M., Taima, K. et al. Effect of Double-Stranded RNA on the Expression of Epithelial Neutrophil Activating Peptide-78/CXCL-5 in Human Endothelial Cells. Inflammation 28, 215–219 (2004). https://doi.org/10.1023/B:IFLA.0000049046.23377.44

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  • DOI: https://doi.org/10.1023/B:IFLA.0000049046.23377.44

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