Abstract
The distribution of the neurosecretory hormones vasotocin, isotocin and melanin-concentrating hormone and the hypophysio-tropic hormone corticotropin-releasing factor was studied in the hypothalamo-hypophyseal system of the white seabream (Diplodus sargus) using immunocytochemical techniques. Magnocellular and parvocellular perikarya immunoreactive for arginine-vasotocin and isotocin were present in the nucleus preopticus. Perikarya immunoreactive for arginine-vasotocin extended more caudally with respect to isotocin-immunoreactive perikarya. Parvocellular perikarya were located at rostroventral levels and magnocellular perikarya in the dorsocaudal portion of the nucleus. Arginine-vasotocin and isotocin did not coexist in the same neuron. Fibres immunoreactive for arginine-vasotocin and isotocin innervated all areas of neurohypophysis and terminate close to corticotropic and melanotropic cells. Perikarya immunoreactive for melanin-concentrating hormone and corticotropin-releasing factor were observed in the nucleus lateralis tuberis, with a few neurons in the nucleus periventricularis posterior. In addition, melanin-concentrating hormone immunoreactive perikarya were detected in the nucleus recessus lateralis. The preoptic nucleus did not show immunoreactivity for these antisera. Fibres showing melanin-concentrating hormone and corticotropin-releasing factor immunoreactivity ended close to the melanotropic and somatolactotrophic cells of the pars intermedia, and close to the corticotrophic cells of the rostral pars distalis.
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Duarte, G., Segura-Noguera, M., Martín del Río, M. et al. The Hypothalamo-hypophyseal System of the White Seabream Diplodus Sargus: Immunocytochemical Identification of Arginine-vasotocin, Isotocin, Melanin-concentrating Hormone and Corticotropin-releasing Factor. Histochem J 33, 569–578 (2001). https://doi.org/10.1023/A:1014912110318
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DOI: https://doi.org/10.1023/A:1014912110318