Log in

Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica)

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

n-Alkanes ranging from C12 to C18 were converted into glycolipid biosurfactants, mannosylerythritol lipids (MEL), by resting cells of Pseudozyma (Candida) antarctica T-34. The highest yield (0.87 g g−1 substrate) was obtained from 6% (v/v) of n-octadecane after 7 days reaction. The amount of MEL reached 140 g l−1 by intermittent feeding of the substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Germany)

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bai G,Brusseau ML,Miller RM (1997) Biosurfactant-enhanced removal of residual hydrocarbon from soil. J. Contam. Hydrol. 25: 157-170.

    Google Scholar 

  • Banat IM,Makkar RS,Cameotra SS (2000) Potential commercial applications of microbial surfactants. Appl. Microbiol. Biotechnol. 53: 495-508.

    Google Scholar 

  • Daniel HJ,Reuss M,Syldatk C (1998) Production of sophorolipids in high concentration from deproteinized whey and rapeseed oil in a two stage fed batch process using Candida bombicola ATCC 22214 and Cryptococcus curvatus ATCC 20509. Biotechnol. Lett. 20: 1153-1156.

    Google Scholar 

  • Kitamoto D,Sangita G,Ourisson G,Nakatani Y (2000) Formation of giant vesicles from diacylmannosylerythritols, and their binding to concanavalin A. Chem. Commun. 2000: 861-862.

    Google Scholar 

  • Kitamoto D,Yanagishita H,Endo A,Nakaiwa M,Nakane T,Akiya T (2001) Remarkable antiagglomeration effect of a yeast biosurfactant, diacylmannosylerythritol, on ice-water slurry for cold thermal storage. Biotechnol. Progr. 17: 362-365.

    Google Scholar 

  • Kitamoto D,Yanagishita H,Haraya K,Kitamoto HK (1998) Contribution of a chain-shortening pathway to the biosynthesis of the fatty acids of mannosylerythritol lipid (biosurfactant) in the yeast Candida antarctica. Biotechnol. Lett. 20: 813-818.

    Google Scholar 

  • Kitamoto D,Yokoshima T,Yanagishita H,Haraya K,Kitamoto HK (1999) Formation of glycolipid biosurfactant, mannosylerythritol lipid, by Candida antarctica from hydrocarbons via subterminal oxidation pathway. J. Oleo Sci. (formerly J. Jpn. Oil Chem. Soc.) 48: 1377-1384.

    Google Scholar 

  • Rattray JBM (1988) Yeasts. In: Ratledge C,Wilkinson SG, eds. Microbial Lipids, Vol. 1. New York: Academic Press, pp. 555-697.

    Google Scholar 

  • van Beilen JB,Wubbolts MG,Witholt B (1994) Genetics of alkane oxidation by Pseudomonas oleovorans. Biodegradation 5: 161-174.

    Google Scholar 

  • Vollbrecht E,Rau U,Lang S (1999) Microbial conversion of vegetable oils into surface-active di-, tri-, and tetrasaccharide lipids (biosurfactants) by the bacterial strain Tsukamurella spec. Fett/Lipid 101: S. 389-394.

    Google Scholar 

  • Wakamatsu Y,Zhao X,** C,Day N,Shibahara M,Nomura N,Nakahara T,Murata T,Yokoyama KK (2001) Mannosylerythritol lipid induces characteristics of neuronal differentiation in PC12 cells through an ERK-related signal cascade. Eur. J. Biochem. 268: 374-383.

    Google Scholar 

  • Zhao X,Wakamatsu Y,Shibahara M,Nomura N,Geltinger C,Nakahara T,Murata T,Yokoyama KK (1999) Mannosylerythritol lipid is a potent inducer of apoptosis and differentiation of mouse melanoma cells in culture. Cancer Res. 59: 482-486.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kitamoto, D., Ikegami, T., Suzuki, G.T. et al. Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica). Biotechnology Letters 23, 1709–1714 (2001). https://doi.org/10.1023/A:1012464717259

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1012464717259

Navigation