Abstract
A new method for the isolation of total RNA from plant tissues is described. This method is designed to circumvent problems resulting from endogenous materials that typically gel during isolation when previously published methods are used. This method is suitable for the isolation of total RNA from plant tissues that contain large amounts of oligosaccharide compounds, and has been used to produce high-quality total RNA from the inner epidermal tissue of onion bulb scale. Using 10 grams of epidermis, it is possible to isolate 0.6 mg of total RNA. This RNA can be directly used in mRNA purification, in vitro translation, and reverse transcription-polymerase chain reaction (RT-PCR). The procedure is simple and takes less than one day.
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Zhou, J., Pesacreta, T.C. & Brown, R.C. RNA Isolation without Gel Formation from Oligosaccharide-Rich Onion Epidermis. Plant Molecular Biology Reporter 17, 397–407 (1999). https://doi.org/10.1023/A:1007650515501
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DOI: https://doi.org/10.1023/A:1007650515501