Abstract
Purpose. Previously, we suggested that alkylglucoside can be aneffective vector for renal-specific drug delivery (Suzuki et al., J. Pharmacol.Exp. Ther., 288:57–61, 1999). The purpose of the present study is tocharacterize the membrane protein which is recognized by thisalkylglucoside.
Methods. The binding of [125I] tyrosine conjugated with aoctylthioglucoside (Glc-S-C8-[125I]Tyr) Glc-S-C8-[125I]Tyr to crude membranefractions of kidney was determined. In addition, the membrane wascross-linked with this alkylglucoside and examined by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
Results. Glc-S-C8-[125I]Tyr was shown to have a specific binding siteon the kidney membrane (Kd = 931 nM and Bmax = 987pmol/mg protein). Cross-linking of the membrane with Glc-S-C8-[125I]Tyrresulted in the detection of a protein (Mr = 62,000), which wasunaffected by reducing agents. The results of this cross-linking study wereconsistent with previous information on its localization and bindingcharacteristics.
Conclusions. The kidney membrane protein, to which alkylglucosidebinds in a specific manner, has a molecular weight of 62,000.Cross-linking is a useful tool for detecting this novel membrane proteinin kidney.
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Watanabe, Y., Suzuki, H., Suzuki, K. et al. Detection of the Membrane Protein Recognized by the Kidney-Specific Alkylglucoside Vector. Pharm Res 17, 49–54 (2000). https://doi.org/10.1023/A:1007566408323
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DOI: https://doi.org/10.1023/A:1007566408323