SpringerLink
Log in

Study on the relationship between calcium-induced calcium release from mitochondria and PTP opening

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

The permeability transition pore (PTP) is central for mitochondria function. PTP either open in low-conductance state to carry out mCICR (Ca2+-induced Ca2+ release from mitochondria) and play roles in cell phsyiological activities or open in high-conductance conformation to release harmful substances and play important roles in cell pathological responses and apoptosis. The results of study on the relationship between mCICR and PTP opening show Ca2+ concentrations but not the Ca2+ delivery mode determined the occurrence of mCICR or PTP opening. Ca2+-induced PTP opening began with and depended on mCICR. mCICR was a prerequisite for H2O2 and As2O3-induced PTP opening. The results indicated that the PTP opening was determined by Ca2+ stimulation intensity but not mode. PTP could switch from low- to high-conductance conformation and the PTP open by high-conductance began with low-conductance state. mCICR is necessary for Ca2+-dependent PTP opening. Our data suggested also that it would be possible to control cellular responses first by modulating mCICR, then by regulating PTP opening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price includes VAT (Brazil)

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Jouaville LS, Ichas F, Mazat J-P: Modulation of cell calcium signals by mitochondria. Mol Cell Chem 184: 371-376, 1998

    Google Scholar 

  2. Babcock DF, Herrington J, Goodwin PC, Park YB, Hille B: Mitochondrial participation in the intracellular Ca2+ network. J Cell Biol 136: 833-844, 1997

    Google Scholar 

  3. Ichas F, Jouaville LS, Sidash SS, Mazat J-P, Holmuhamedov EL: Mitochondrial calcium spiking: A transduction mechanism based on calciuminduced permeability transition involved in cell calcium signaling. FEBS Lett 348: 211-215, 1994

    Google Scholar 

  4. Ichas F, Jouaville LS, Mazat J-P: Mitochondria are excitable organelles capable of generating and conveying electrical and calcium signals. Cell 89: 1145-1153, 1997

    Google Scholar 

  5. Griffiths EJ, Halestrup AP: Protection by cyclosporin A of ischemia/reperfusion-induced damage in isolated rat hearts. J Mol Cell Cardiol 25: 1461-1469, 1993

    Google Scholar 

  6. Trost LC, Lemasters JJ: The mitochondrial permeability transition: A new pathophysiological mechanism for Reye's syndrome and toxic liver injury. J Pharmacol Exp Ther 278: 1000-1005, 1996

    Google Scholar 

  7. Schinder AF, Olson EC, Spitzer NC, Montal M: Mitochondria dysfunction is a primary event in glutamate neurotoxicity. J Neurosci 16: 6125-6133, 1996

    Google Scholar 

  8. Susin SA, Zamzami N, Kroemer G: Mitochondria as regulators of apoptosis: Doubt no more. Biochim Biophys Acta 1366: 151-165, 1998

    Google Scholar 

  9. Green DR, Reed JC: Mitochondria and apoptosis. Science 281: 1309-1312, 1998

    Google Scholar 

  10. Ichas F, Mazat J-P: From calcium signaling to cell death: Two conformations for the mitochondrial permeability transition pore. Switching from low-to high-conductance state. Biochim Biophys Acta 1366: 33-50, 1998

    Google Scholar 

  11. Gunter TE, Gunter KK, Sheu S-S, Garvin CE: Mitochondrial calcium transport: Physiological and pathological relevance. Am J Physiol 267: C313-C319, 1994

    Google Scholar 

  12. Jouaville LS, Ichas F, Mazat J-P: Modulation of cell calcium signals by mitochondria. Mol Cell Biochem 184: 371-376, 1998

    Google Scholar 

  13. Lemasters JJ, Nieminen A-L, Qian T, Trost LC, Elmore SP, Nishimura Y, Crowe RA, Cascio WE, Bradham CA, Brenner DA, Herman B: The mitochondrial permeability transition in cell death: A common mechanism in necrosis, apoptosis and autophagy. Biochim Biophys Acta 1366: 177-196, 1998

    Google Scholar 

  14. Zoratti M, Szabo I: The mitochondrial permeability transition. Biochim Biophys Acta 1241: 139-176, 1995

    Google Scholar 

  15. Broekemeier KM, Klocek CK, Pferfer DR: Proton selective substate of mitochondrial permeability pore: Regulation by the redox state of the electron transport chain. Biochemistry 37: 13059-13065, 1998

    Google Scholar 

  16. Frei B, Winterhalter KH, Richter C: Mechanism of alloxan-induced calcium release from liver mitochondria. J Biol Chem 260: 7394-740, 1985

    Google Scholar 

  17. Petronilli V, Szabo I, Zoratti M: The inner mitochondrial membrane contains ion conducting channels similar to those found in bacteria. FEBS Lett 259: 137-143, 1989

    Google Scholar 

  18. Emaus RK, Grunwald R, Lemasters JJ: Rhodamine123 as a probe of transmembrane potential in isolated rat-liver mitochondria: Spectral and metabolic properties. Biochim Biophys Acta 850: 436-448, 1986

    Google Scholar 

  19. Babcock DF, Hille B: Mitochondrial oversight of cellular Ca2+ signaling. Curr Opin Neurobiol 8: 398-404, 1998

    Google Scholar 

  20. Hoyt KR, Sharma TA, Reynolds I: Trifluoperazine and dibucaine-induced inhibition of glutamate-induced mitochondria depolarization in rat cultured forebrain neurons. Br J Pharmacol 122: 803-808, 1997

    Google Scholar 

  21. Larochette N, Decaudin D, Jacotot E, Brenner C, Marzo I, Susin SA, Zamzami N, **e Z, Reed J, Kroemer G: Arsenite induces apoptosis via a direct effect on the mitochondrial permeability transition pore. Exp Cell Res 249: 413-421, 1999

    Google Scholar 

  22. Kowaltowski AJ, Vercesi AE, Castilho RF: Mitochondrial membrane protein thiol reactivity with N-ethylmaleimide or mersalyl is modified by Ca2+: Correlation with mitochondrial permeability transition. Biochim Biophys Acta 1318: 395-402, 1997

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Bei**g, 100101, China

    **ngxu Huang

  2. National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Bei**g, 100101, China

    Dayong Zhai & Youguo Huang

Authors
  1. **ngxu Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dayong Zhai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Youguo Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, X., Zhai, D. & Huang, Y. Study on the relationship between calcium-induced calcium release from mitochondria and PTP opening. Mol Cell Biochem 213, 29–35 (2000). https://doi.org/10.1023/A:1007138818124

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1007138818124

Search

Navigation