Abstract
In this study, we characterized Hypericum vesiculosum Griseb. (sect. Drosocarpium), Hypericaceae, a Balkan endemic and Greek subendemic species, which was collected from the Peloponnese (Greece). The anatomical study showed that its leaves are dorsiventral and hypostomatic, and the major part of its circular stem is filled with xylem. High-performance liquid chromatography coupled with diode-array detection and mass spectrometry analysis of the methanolic extract of the aerial parts revealed 25 compounds, and the concentration of the 18 major ingredients was determined with a validated method of liquid chromatography coupled with diode-array detection. Flavonoids were the most abundant group of compounds (> 100 mg/g of dry extract) with I3,II8-biapigenin, apigenin hexoside, isoquercitrin, quercetin, and hyperoside as the major dereplicated constituents. Chlorogenic acid was abundant (9.34 ± 0.22 mg/g) and the content of naphthodianthrones was comparable to that in H. perforatum. Only one phloroglucinol could be quantified at a very low concentration and that was not hyperforin. This is the first chemical analysis of its methanolic extract and anatomical characterization of its vegetative tissues.
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Acknowledgments
The authors are grateful to the Laboratory of Plant Physiology, Department of Biology, University of Patras for giving access to their equipment (Zeiss Axioplan light microscope).
Funding
The authors would like to acknowledge the financial support by the project “PlantUP” (MIS5002803), which is implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Programme “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014–2020) and co-financed by Greece and the European Union (European Regional Development Fund).
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All authors contributed to the study conception and design. KZ and GI collected the plant material and GI identified it. APV and KZ extracted the samples and carried out the HPLC analysis. KZ performed the LC-MS analysis, and together with DK the anatomical analysis. FNL supervised the chemical analysis and GI the morphological one. KZ, APV, and FNL were in charge of writing the manuscript. DK and GI provided critical reading and insightful recommendations. All the authors have read the final manuscript and approved the submission.
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Zeliou, K., Vogiatzoglou, A.P., Kalachanis, D. et al. Anatomical Characterization and UHPLC-MS Analysis of Hypericum vesiculosum. Rev. Bras. Farmacogn. 30, 416–422 (2020). https://doi.org/10.1007/s43450-020-00062-0
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DOI: https://doi.org/10.1007/s43450-020-00062-0