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Development of dual stress-tolerant rice variety Jyothi with Saltol and Sub1 genes through marker-assisted backcross breeding

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Abstract

Abiotic stresses such as salinity, submergence, drought and extreme temperatures greatly affect rice production worldwide. Marker-assisted selection was effectively used to introgress QTLs for salinity tolerance (Saltol) and submergence tolerance (Sub1) into the background of elite rice variety Jyothi. Saltol introgressed BC1F2 lines of Jyothi with 79.3% of recipient parent genome was crossed with Sub1 introgressed BC2F2 lines of Jyothi showing 95.9% of parent genome recovery. F1 progenies were screened with Saltol and Sub1 linked foreground markers and recombinant markers. F1 heterozygous plants were selfed to produce F2 generation. F2 progenies homozygous for both the loci were selected. Phenotypic screening for salinity and submergence tolerance was performed to validate the introgressed genes. The plants able to tolerate both salinity and submergence stresses were selected for selfing to raise F3 progenies. The pyramided lines were also similar to recurrent parent in agro-morphological and grain quality traits.

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Authors and Affiliations

  1. Rice Research Station, Kerala Agriculture University, Vyttila, Kerala, India

    Deepa John, P. C. Rohini & K. S. Shylaraj

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  1. Deepa John
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  2. P. C. Rohini
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  3. K. S. Shylaraj
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Correspondence to Deepa John.

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Communicated by P. Stephen Baenziger.

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John, D., Rohini, P.C. & Shylaraj, K.S. Development of dual stress-tolerant rice variety Jyothi with Saltol and Sub1 genes through marker-assisted backcross breeding. CEREAL RESEARCH COMMUNICATIONS (2023). https://doi.org/10.1007/s42976-023-00432-z

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