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Electrochemical field-effect bio-transistor based on a multi-scale electronic nanomesh of single-walled carbon nanotubes

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Abstract

Single-walled carbon nanotube (SWNT) has gained significant interest as a transducer in various electrochemical sensing devices due to their unique structure, compatibility with biomolecules, and excellent electronic properties. As-prepared SWNTs are usually a mixture of semiconducting and metallic ones. Despite of the higher content of semiconducting components in mixed SWNTs, metallic properties are predominantly expressed due to the bundling issue of the SWNT during the fabrication process, limiting the applicability to bio-transistor application. Here, we present a multi-scale semiconducting electronic film of SWNTs as a transducing platform for electrochemical field-effect-transistor (eFET) suitable for the sensitive detection of subtle biological modulation. A genetically engineered filamentous M13 phage showing strong binding affinity toward SWNTs on its body surface was used as a biological material, allowing us to fabricate a large-scale transparent semiconducting nanocomposite. As the relative ratio of SWNT to M13 phage decreases, the on–off ratio of SWNT electronic film increases by 1200%. To show broad applicability, the multi-scale SWNT nanomesh-based eFET is applied for monitoring a variety of biological reactions in association with enzymes, aptamers, and even cyanobacteria. The biomimetic electronic material system with the capability of transducing biological responses at a large scale over a broad dynamic range holds excellent promise for biosensors, biofuel cells, and environment monitoring.

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Acknowledgements

This study was financially supported by Seoul National University of Science and Technology (SEOULTECH).

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Authors and Affiliations

  1. Department of Fine Chemistry, Seoul National University of Science and Technology, 232 Gongneung-Ro, Nowon-gu, Seoul, 01811, Korea

    Seung-Woo Lee, Ki-Sung Cheon, Hyunseok Lee & Kyowook Hwang

  2. Department of Microbiology, Pusan National University, 2 Busandaehak‐ro 63 Beon‐gil, Geumjeong‐gu, Busan, 46241, Korea

    Eun-Hee Lee

  3. Center for Functional Biomaterials, Seoul National University of Science and Technology, 232 Gongneung‐ro, Nowon‐gu, Seoul, 01811, Korea

    Seung-Woo Lee

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  1. Seung-Woo Lee
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Lee, SW., Cheon, KS., Lee, H. et al. Electrochemical field-effect bio-transistor based on a multi-scale electronic nanomesh of single-walled carbon nanotubes. Carbon Lett. 33, 1197–1204 (2023). https://doi.org/10.1007/s42823-023-00485-4

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  • DOI: https://doi.org/10.1007/s42823-023-00485-4

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