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Endophytic bacterial diversity in the latex-bearing caulosphere of Hevea brasiliensis Müll. Arg

  • Environmental Microbiology - Short Communication
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Abstract

Rubber trees are a commercial cash crop, and the milky latex or polyisoprene they produce is the natural source of rubber. Little is known about the bacterial populations found in active zone of latex-bearing caulosphere. We employed a tailored cloud microbial bioinformatic approach for the identification and potential hypothetical ecological roles of an uncultured endophytic hidden bacterial community in the active zone of the latex-bearing caulosphere of Hevea brasiliensis. Small pieces of slivers were collected from healthy plant from the village: Belonia, South Tripura, rubber plantation in Northeastern India. These uncultured bacteria were identified using the V3–V4 hypervariable amplicon region of the 16 S rDNA gene. A total of 209,586 contigs have been generated. EasyMAP Version 1.0, a cloud-based microbial bioinformatics tool with an integrated QIIME2 pipeline, was used to analyze contigs. We detected 15 phyla and 91 OTUs (operational taxonomic units). Proteobacteria (73.5%) was the most enriched phylum, followed by Firmicutes (13.8%), Bacteroidetes (5.2%), and Actinobacteria (3.2%). Ammonia oxidizers, sulfate reducers, dehalogenation, chitin degradation, nitrite reducers, and aromatic hydrocarbon degraders were the most prevalent functional categories in the active zones of caulosphere. Furthermore, Gammaproteobacteria (49.2%) and Erwinia (29.19%) were the most abundant classes and genera of endophytic bacterial communities. Thus, the presence of a substantial amount of phosphate-solubilizing Gammaproteobacteria (PSB) may stimulate growth, increase plant resilience, suppress disease, and aid in the rubber and sugar breakdown. This is the first report of microbial endophytes associated with Hevea caulosphere.

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Data availability

The total size of the amplicon metagenomic sequence reads is 79.4 Mb, including 209, 586 fastq paired-end reads and 126 M bases. The amplicon reads were available in the NCBI-Sequence Read Archive with accession number: SRX20114400 and BioProject ID: PRJNA962253.

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Acknowledgements

The authors express their gratitude to the Principal of Bangabasi Evening college, Principal of Narasinha Dutta College, Howrah, West Bengal and Head of Department of Botany, DSPMU, Ranchi, Jharkhand for provide all the necessary facilities and laboratory usage. Dr. Prasanta Dey, Postdoctoral Fellow, Division of Medical Oncology, Rutgers Cancer Institute of New Jersey, USA and Ratan Dey from Tripura deserve special gratitude for his assistance during the survey and sample collection.

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Author notes

  1. Vineet Vishal and Tandra Das contributed equally to this work.

Authors and Affiliations

  1. Department of Botany, Narasinha Dutta College, Howrah, West Bengal, 711101, India

    Tandra Das

  2. Department of Botany, Bangabasi Evening College, Kolkata, West Bengal, 700009, India

    Vineet Vishal & Sabdar Rahaman

  3. Department of Botany, Dr. Shyama Prasad Mukherjee University, Ranchi, Jharkhand, 834008, India

    Vineet Vishal & Shalini Lal

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  1. Vineet Vishal
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Contributions

The ideas and project were developed by VV, TD, SL and SR. VV, TD, and SR, were involved in the research and investigation process, particularly in experimentation. TD, VV, SL and SR have equally contributed in data interpretation, analysis and writing of the manuscript. All of the authors provided constructive feedback on how to implement the study, analysis, and develo** the article. The version to be submitted, as well as any revised versions, received final approval from SR.

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Correspondence to Sabdar Rahaman.

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Vishal, V., Das, T., Lal, S. et al. Endophytic bacterial diversity in the latex-bearing caulosphere of Hevea brasiliensis Müll. Arg. Braz J Microbiol (2024). https://doi.org/10.1007/s42770-024-01373-3

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