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Virulence factors and antimicrobial resistance of Escherichia coli isolated from commercialized fresh cheese in the south of Espírito Santo

  • Food Microbiology - Research Paper
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Abstract

Cheeses are dairy products that can potentially contain a diverse range of harmful bacteria that could be consumed by humans, including the enteric pathogen Escherichia coli. This study aimed to characterize the presence of total coliforms, assess the antimicrobial susceptibility profiles of the main commercial antimicrobial classes and biocides, and evaluate the ability of 50 E. coli isolates obtained from fresh cheese sold in the southern region of Espírito Santo, Brazil, to produce biofilms. The counts of total coliforms + E. coli obtained averages of (A) 7.22 × 106 CFU/g, (B) 9.35 × 107 CFU/g, and (C) 1.16 × 106 CFU/g for different brands. All isolates were capable of forming biofilms, with 8%, 76%, and 16% of these isolates presenting high, moderate, and low adherence in biofilm formation, respectively. Most strains showed inhibition halos for the biocides chlorhexidine digluconate 2% (16 mm ± 4.34), iodopovidone 10% (7.14 mm ± 0.36), and sodium hypochlorite 2% (7.12 mm ± 0.33). Out of the 50 strains, 21 (42%) were resistant to at least one of the antimicrobials. Regarding the multiple resistance index, 3 (6%) strains were resistant to 3 or more antimicrobial classes. Furthermore, 2 (4%) were extended-spectrum beta-lactamases producers. Resistance to ampicillin and amoxicillin was observed in 20% and 40% of the strains, respectively. In contrast, gentamicin was the most effective antimicrobial, with a sensitivity rate of 100%. The findings indicate that E. coli present in fresh cheese may possess unique physiological characteristics that could be associated with their persistence, virulence, and multidrug resistance. These results raise significant public health concerns since contaminated food can pose risks to consumers’ health, emphasizing the importance of reinforcing hygienic-sanitary controls at all stages of production.

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Acknowledgements

This study was supported by Universidade Federal do Espírito Santo (UFES), Fundação de Amparo à Pesquisa e Inovação do Espírito Santo; Conselho Nacional de Desenvolvimento Científico e Tecnológico; and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (financing modality 001).

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  1. Graduate Program in Veterinary Sciences, Federal University of Espírito Santo (UFES), Alegre, ES, Brazil

    Kássia Vidal Menezes, Nicolly Soares Ferreira & Juliana Alves Resende

  2. Department of Pharmacy and Nutrition, Federal University of Espirito Santo (UFES), Alto Universitário; S/N, Alegre, ES, 29500-000, Brazil

    Bruna Maria Fia Pimentel, Joyce Aparecida Corrêa Da Costa, Mariana Drummond Costa Ignacchiti & Juliana Alves Resende

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Menezes, K.V., Pimentel, B.M.F., Da Costa, J.A.C. et al. Virulence factors and antimicrobial resistance of Escherichia coli isolated from commercialized fresh cheese in the south of Espírito Santo. Braz J Microbiol 54, 2063–2071 (2023). https://doi.org/10.1007/s42770-023-01013-2

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