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Anti-inflammatory potential of Lactiplantibacillus plantarum IDCC 3501 and its safety evaluation

  • Food Microbiology - Research Paper
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Abstract

This study investigated the anti-inflammatory activity of Lactiplantibacillus plantarum IDCC 3501 isolated from kimchi (Korean fermented food) and its safety. When lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were treated with cell-free supernatant from L. plantarum IDCC 3501, the mRNA expression level of inflammatory markers (i.e., TNF-α, IL-1β, and IL-6) was significantly reduced. In addition, the decreased cell viability by LPS was recovered and NO production in LPS-induced cell was also decreased. For the safety assessment, the genes responsible for antibiotic resistance and virulence were not detected from the genome analysis of this strain. Consistent with this, minimal inhibitory concentrations against various antibiotics, biogenic amines, and d-lactate production, as well as enzymatic and hemolysis activities, indicated that L. plantarum IDCC 3501 did not produce any harmful compounds during fermentation. Furthermore, no acute toxicity and mortality were observed in a murine mouse model. Based on our findings, L. plantarum IDCC 3501 is safe and beneficial for human consumption.

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All data generated or analyzed during this study are included in this published article and its additional information files.

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Funding

This work was supported by Ildong Bioscience and the National Research Foundation of Korea (NRF) funded by Korea government (Ministry of Science and ICT; Grant No. 2020R1C1C1005251).

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Authors and Affiliations

  1. Ildong Bioscience, 17 Poseunggongdan-ro, Pyeongtaek-si, Gyeonggi-do, 17957, Republic of Korea

    Soo-Yeon Yang, Seung A Chae, Won Yeong Bang, Minjee Lee, O-Hyun Ban & Jungwoo Yang

  2. School of Food Science and Biotechnology, Kyungpook National University, Daegu, 41566, Republic of Korea

    O-Hyun Ban & Young Hoon Jung

  3. Department of Integrative Food Bioscience and Biotechnology, Chonnam National University, Gwangju, 61186, Republic of Korea

    Soo-Jung Kim

Authors
  1. Soo-Yeon Yang
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  2. Seung A Chae
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  4. Minjee Lee
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Contributions

S-Y Yang, WY Bang, and SA Chae carried out the in vitro experiments and S-Y Yang drafted the manuscript. SA Chae and M Lee carried out the cell experiments. O–H Ban carried out the genetic studies, S-J Kim participated in the design of the study, and YH Jung and J Yang conceived the study and participated in its design and coordination, and finalized the manuscript. All authors read and approved the final manuscript.

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Correspondence to Young Hoon Jung or Jungwoo Yang.

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Yang, SY., Chae, S.A., Bang, W.Y. et al. Anti-inflammatory potential of Lactiplantibacillus plantarum IDCC 3501 and its safety evaluation. Braz J Microbiol 52, 2299–2306 (2021). https://doi.org/10.1007/s42770-021-00603-2

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  • DOI: https://doi.org/10.1007/s42770-021-00603-2

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