Abstract
In the present study, we synthesis nanoparticles using biosynthesis methods because of the eco-friendly approach. Gold nanoparticles were synthesized using extracellular metabolites of marine bacteria (Rastrelliger kanagurta, Selachimorpha sp., and Panna microdon). After the synthesis gold nanoparticles checked their antibacterial and antimycobacterial activities. Here we have few techniques that have been used for characterizing the gold nanoparticles followed by ultraviolet (UV)-visible spectrophotometer analysis, Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscopy (TEM). We observed the formation of gold nanoparticles using UV-Vis spectroscopy (UV-Vis). FT-IR spectroscopy results of the extracellular metabolites showed that different characteristic functional groups are responsible for the bioreduction of gold ions. In the recent years, we used zebrafish for an animal model to estimate nanoparticle toxicity and biocompatibility. We tested toxicity of the gold nanoparticle using the zebrafish larvae that are growing exponentially. Sample 1 showed a good antimicrobial activity, and sample 5 showed a good antimycobacterial activity. Based on the UV spectrophotometer, sample 1 is used for further studies. Color change and UV spectrum confirmed gold nanoparticles. Based on the TEM and SEM particles, size was measured and ranged between 80 and 45 nm, and most of the particles are spherical and are in rod shape. XRD result showed the gold nanoparticles with crystalline nature. Toxicity studies in the zebrafish larvae showed that 50 μg ml−1 showed less toxicity. Based on the studies, gold nanoparticle has good antibacterial and antimycobacterial activities. The present was concluded that gold nanoparticles have potential biocompatibility and less toxicity. Gold nanoparticles will be used as a drug molecule in pharmaceutical company and biomedicine application.
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References
Davis MH (2018) Empathy: a social psychological approach. Routledge
Betts JW, Hornsey M, La Ragione RM (2018) Novel antibacterial: alternatives to traditional antibiotics. AdvMicrob Physiol 73:123–169
Blecher K, Nasir A, Friedman A (2011) The growing role of nanotechnology in combating infectious disease. Virulence. 2(5):395–401
Singh P, Chauhan NR, Rajesha S (2019) Influence of cobalt, iron and copper on microstructure and mechanical properties of alumina/SiC nano-ceramic composite. Mater Res Express 6(6):065027
Vazquez E, Villaverde A (2010) Engineering building blocks for self-assembling protein nanoparticles. Microb Cell Factories 9(1):101
Camas M, Celik F, Sazak Camas A, Ozalp HB (2019) Biosynthesis of gold nanoparticles using marine bacteria and box–Behnken design optimization. Part Sci Technol 37(1):31–38
Jayaramudu T, Raghavendra GM, Varaprasad K, Sadiku R, Raju KM (2013) Carbohydr Polym 92:2193
Nangia Y, Wangoo N, Sharma S, Wu J, Dravid V (2009) J ApplPhys Lett 94:233901
Huang X, Neretina S, EI-Sayed MA (2009) Adv Mater 21:4880
Jenkins JA, Bart HL Jr, Bowker JD, Bowser PR, MacMillan JR, Nickum JG, Rachlin JW, Rose JD, Sorensen PW, Warkentine BE, Whitledge GW (2014) Guidelines for use of fishes in research-revised and expanded. Fisheries. 39(9):415–416
Yang L, Wang Y, Zhang Z, He S (2014) Comprehensive transcriptome analysis reveals accelerated genic evolution in a Tibet fish, Gymnodiptychuspachycheilus. Genome Biol Evol 7(1):251–261
Agnihotri M, Joshi S, Kumar AR, Zinjarde S, Kulkarni S (2009) Biosynthesis of gold nanoparticles by the tropical marine yeast Yarrowialipolytica NCIM 3589. Mater Lett 63(15):1231–1234
National Committee for Clinical Laboratory Standards (1993) Performance standards for antimicrobial disk susceptibility tests. Approved standard. NCCLS document M2-A5. Wayne, Pa: National Committee for Clinical Laboratory Standards
Sivaraj A, Kumar V, Sunder R et al (2019) Commercial yeast extracts mediated green synthesis of silver chloride nanoparticles and their anti-mycobacterial Activity. J Clust Sci. https://doi.org/10.1007/s10876-019-01626-4
Bankar AV, Kumar AR, Zinjarde SS (2009) Removal of chromium (VI) ions from aqueous solution by adsorption onto two marine isolates of Yarrowialipolytica. J Hazard Mater 170(1):487–494
Pourali P, Baserisalehi M, Afsharnezhad S, Behravan J, Alavi H, Hosseini A (2012) Biological synthesis of silver and gold nanoparticles by bacteria in different temperatures (37°C and 50 C). J Pure Appl Microbiol 6:757–763
Caroling G, Tiwari SK, Ranjitham AM, Suja R (2013) Biosynthesis of silver nanoparticles using aqueous broccoli extract-characterization and study of antimicrobial, cytotoxic effects. Asian J Pharm ClinRes 6(4):165–172
Westerfield M (2000) The zebrafish book. A guide for the laboratory use of zebrafish (Daniorerio). Oregon press, Eugene
Luo Z, Zheng K, **e J (2014) Engineering ultrasmall water-soluble gold and silver nanoclusters for biomedical applications. Chem Commun 50(40):5143–5155
Khan AK, Rashid R, Murtaza G, Zahra A (2014) Gold nanoparticles: synthesis and applications in drug delivery. Trop J Pharm Res 13(7):1169–1177
Borm PJA, Müller-Schulte D (2006) Nanoparticles in drug delivery and environmental exposure: same size, same risks? Nanomedicine:235–249
Joseph BM (2015) Microwave assisted facile green synthesis of silver and gold nanocatalysts using the leaf extract of Aervalanata. Spectrochim Acta A Mol Biomol Spectrosc 136:1371–1379
Solano-Umaña V, Vega-Baudrit RJ (2015) Gold and silver nanotechology on medicine. J ChemBiochem 3(1):21–33
SoltaniNejad M, ShahidiBonjar GH, Khaleghi N (2015) Biosynthesis of gold nanoparticles using streptomycesfulvissimus isolate. Nanomedicine J 2(2):153–159
Biradar L (2012) Isolation and screening of gold nanoparticles by microbes. World J Sci Technol:20–22
Zhang X, Qu Y, Shen W, Wang J, Li H, Zhang Z, Li S, Zhou J (2016) Biogenic synthesis of gold nanoparticles by yeast Magnusiomycesingens LH-F1 for catalytic reduction of nitrophenols. Colloids Surf A Physicochem Eng Asp 497:280–285
Vanaja M, Paulkumar K, Gnanajobitha G, Rajeshkumar S, Malarkodi C (2014) Annadurai G (2014) herbal plant synthesis of antibacterial silver nanoparticles by Solanumtrilobatum and its characterization. Int J Met
Rajasree SR, Suman TY (2012) Extracellular biosynthesis of gold nanoparticles using a gram negative bacterium Pseudomonas fluorescens. Asian Pacific Journal of Tropical Disease 2:S796–S799
Correa-Llanten DN, Muñoz-Ibacache SA, Castro ME, Muñoz PA, Blamey JM (2013) Gold nanoparticles synthesized by Geobacillus sp. strain ID17 a thermophilic bacterium isolated from Deception Island, Antarctica. Microb Cell Fact 12(1):75
Kikuchi F, Kato Y, Furihata K, Kogure T, Imura Y, Yoshimura E, Suzuki M (2016) Formation of gold nanoparticles by glycolipids of Lactobacillus casei. Sci Rep 6:34626
**a T, Li N, Nel AE (2009) Potential health impact of nanoparticles. Annu Rev Public Health 30:137–150
Bakri SJ, Pulido JS, Mukherjee P, Marler RJ, Mukhopadhyay D (2008) Absence of histologic retinal toxicity of intravitrealnanogold in a rabbit model. Retina. 28:147–149
Zhu X, Zhu L, Duan Z, Qi R, Li Y, Lang Y (2008) Comparative toxicity of several metal oxide nanoparticle aqueous suspensions to zebrafish (Daniorerio) early developmental stage. J Environ Sci Health Tox Haz Subst Environ (Eng) 43:278–284
AshaRani PV, Low Kah MG, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3:279–290
Bar-Ilan O, Albrecht RM, Fako VE, Furgeson DY (2009) Toxicity assessments of multisized gold and silver nanoparticles in zebrafish embryos. Small 5:1897–1910
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The authors are grateful to the Science and Engineering Research Board (SERB-Early Career Research Award ECR/2015/000460), Government of India, New Delhi, India for the financial support and also thankful to the Management of Sathyabama Institute of Science and Technology (Deemed University) Chennai, Tamil Nadu, India, for providing the Infrastructure facility.
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Rajasekar, T., Karthika, K., Muralitharan, G. et al. Green synthesis of gold nanoparticles using extracellular metabolites of fish gut microbes and their antimicrobial properties. Braz J Microbiol 51, 957–967 (2020). https://doi.org/10.1007/s42770-020-00263-8
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DOI: https://doi.org/10.1007/s42770-020-00263-8