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Analysis of culturable microbiota present in the stomach of children with gastric symptoms

  • Clinical Microbiology - Research Paper
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Abstract

Despite extensive studies on the gastric microbiota, including Helicobacter pylori and non-H. pylori, the bacterial composition in children remains unknown. In this study, we analyzed the culturable gastric bacteria in stomach biopsies from 346 children aged 1–15 years affected by gastric diseases. H. pylori and non-H. pylori were identified by specific PCR and 16S rDNA sequencing, respectively. Antibiotic susceptibilities of H. pylori and non-H. pylori were tested by the E-test and disk diffusion methods, respectively. Rapid diagnosis was also performed by H. pylori–specific PCR. Twenty-two H. pylori strains were obtained from culture, and 92 biopsies were positive by H. pylori–specific PCR. The positive rate was higher in boys (40.3%) than in girls (23.3%) (P = 0.001). Resistance rates of 22 H. pylori strains were as follows: metronidazole, 86.4%; tetracycline, 22.7%; amoxicillin, 22.7%; levofloxacin, 31.8%; clarithromycin, 36.4%. Ten isolates were multidrug-resistant. Additionally, among 366 non-H. pylori strains, 204 exhibited urease activity. Non-H. pylori resistance rates were as follows: metronidazole, 94.8%; tetracycline, 26.2%; amoxicillin, 42.6%; levofloxacin, 15.3%; clarithromycin, 46.7%. Our results showed that children with gastric disorders harbor stomach bacteria with urease activity or nitrate reductase activity. Further studies will determine the effects of non-H. pylori bacteria in gastric diseases.

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Acknowledgements

We would like to thank Editage [www.editage.cn] for the English language editing.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81460314 and 3176080034), Foundation of Education Department of Guizhou (Project number: KY-2014-216), and Foundation of Guiyang Health and Family Planning Commission (No. 2014-018).

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  1. Changcheng Guo, Fang Liu and Li Zhu contributed equally to this work.

Authors and Affiliations

  1. School of Basic Medical Science, Key Laboratory of Medical microbiology and parasitology of Education Department of Guizhou, Guizhou Medical University, No.1 South Dongqing Road, Guiyang, 550025, China

    Changcheng Guo, Fang Liu, Fangcao Wu, Guzhen Cui, Qiong Wang, Lin Yin, Caixia Wang, Huan Wang, **aojuan Wu, Zhengrong Zhang & Zhenghong Chen

  2. Clinical Medical School of Maternal and Child Affiliated to Guizhou Medical University, No.63 South Rui** Road, Guiyang, 550003, Guizhou, China

    Li Zhu & Yan **ong

  3. Department of Microbiology, School of Basic Medical Science, Guizhou Medical University, Guiyang, China

    Zhenghong Chen

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  1. Changcheng Guo
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Guo, C., Liu, F., Zhu, L. et al. Analysis of culturable microbiota present in the stomach of children with gastric symptoms. Braz J Microbiol 50, 107–115 (2019). https://doi.org/10.1007/s42770-018-0030-5

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