Abstract
Interferon-γ is a kind of protein with a wide range of biological activities, which can regulate the immune function of the body, and can be used as an important marker to detect and treat bovine tuberculosis diseases. Here, a picogram-level bovine interferon-γ (BoIFN-γ) electrochemical impedance immunosensor was constructed for the first time using mesoporous silica nanospheres (MSNs) to immobilize specific monoclonal BoIFN-γ antibodies. The MSNs and BoIFN-γ immunosensors were characterized using scanning electron microscopy, transmission electron microscope, nitrogen adsorption experiment, X-ray photoelectron spectra, and contact angle measurements. MSNs possess a substantial specific surface area and significant hydrophilicity, and can immobilize many antibody molecules, thereby improving detection sensitivity. The immunosensor has a linear detection range from 0.001 to 10.0 ng/mL with an exceptionally low detection limit of 0.62 pg/mL. Compared to the traditional BoIFN-γ analysis method, BoIFN-γ immunosensor presents superiorities in sensitivity, wide linear range as well as short processing time. More importantly, the BoIFN-γ sensor exhibits high selectivity, reliable repeatability as well as stability, providing a promising application prospect for the early diagnosis of Mycobacterium bovis infection.
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Data availability
The data supporting the finding reported herein are available on reasonable request from the corresponding author.
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Acknowledgements
This research was funded by by National Key Research and Development Program of China (2021YFD1800403), National Natural Science Foundation of China (21475116, 21575125 and 81302016), Natural Science Foundation of Jiangsu Province (BK20221370, BK20221281), Key University Natural Science Foundation of Jiangsu Province (20KJA150004), the Project for Science and Technology of Yangzhou (YZ2022074, YZ2020076), Cross-cooperation project of Subei Peoples' Hospital of Jiangsu Province (SBJC220009), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX21_3203).