Abstract
We evaluated the influence of the open porosity of alumina (Al2O3) substrates on the phase formation of calcium phosphates deposited onto it surface. The Al2O3 substrates were prepared with different porosities by the foam-gelcasting method associated with different amounts of polyethylene beads. The substrates were coated biomimetically for 14 and 21 days of incubation in a simulated body fluid (SBF). Scanning electron microscopy characterisation and X-ray computed microtomography showed that the increase in the number of beads provided an increase in the open porosity. The X-ray diffraction and infrared spectroscopy showed that the biomimetic method was able to form different phases of calcium phosphates. It was observed that the increase in the porosity favoured the formation of β-tricalcium phosphate for both incubation periods. The incubation period and the porosity of the substrates can influence the phases and the amount of calcium phosphates formed. Thus, it is possible to target the best application for the biomaterial produced.
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References
Abe Y, Kokubo T, Yamamuro T (1990) Apatite coating on ceramics, metals and polymers utilizing a biological process. J Mater Sci Mater Med 1:233–238. https://doi.org/10.1007/BF00701082
Annabi N, Nichol JW, Zhong X et al (2010) Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Tissue Eng Part B Rev 16:371–383. https://doi.org/10.1089/ten.teb.2009.0639
Arabnejad S, Burnett Johnston R, Pura JA et al (2016) High-strength porous biomaterials for bone replacement: a strategy to assess the interplay between cell morphology, mechanical properties, bone ingrowth and manufacturing constraints. Acta Biomater 30:345–356. https://doi.org/10.1016/j.actbio.2015.10.048
Arjunan A, Baroutaji A, Robinson J et al (2022) Future directions and requirements for tissue engineering biomaterials. Encyclopedia of smart materials. Elsevier, Amsterdam, pp 195–218
ASTM D3967-95a (2001) Splitting Tensile Strength of Intact Rock Core Specimens. ASTM Int
Barrere F, van Blitterswijk C, de Groot K, Layrolle P (2002a) Influence of ionic strength and carbonate on the Ca-P coating formation from SBF×5 solution. Biomaterials 23:1921–1930. https://doi.org/10.1016/S0142-9612(01)00318-0
Barrere F, van Blitterswijk C, de Groot K, Layrolle P (2002b) Nucleation of biomimetic Ca–P coatings on Ti6Al4V from a SBF×5 solution: influence of magnesium. Biomaterials 23:2211–2220. https://doi.org/10.1016/S0142-9612(01)00354-4
Belwanshi M, Jayaswal P, Aherwar A (2021) A study on tribological effect and surface treatment methods of Bio-ceramics composites. Mater Today Proc 44:4131–4137. https://doi.org/10.1016/j.matpr.2020.10.458
Bohner M, Santoni BLG, Döbelin N (2020) β-tricalcium phosphate for bone substitution: synthesis and properties. Acta Biomater 113:23–41. https://doi.org/10.1016/j.actbio.2020.06.022
Böke F, Schickle K, Fischer H (2014) Biological activation of inert ceramics: recent advances using tailored self-assembled monolayers on implant ceramic surfaces. Materials (basel) 7:4473–4492. https://doi.org/10.3390/ma7064473
Brangule A, Gross KA (2015) Importance of FTIR spectra deconvolution for the analysis of amorphous calcium phosphates. IOP Conf Ser Mater Sci Eng. https://doi.org/10.1088/1757-899X/77/1/012027
Canal C, Ginebra MP (2011) Fibre-reinforced calcium phosphate cements: a review. J Mech Behav Biomed Mater 4:1658–1671. https://doi.org/10.1016/j.jmbbm.2011.06.023
Chen Q, Zhu C, Thouas GA (2012) Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites. Prog Biomater 1:2. https://doi.org/10.1186/2194-0517-1-2
Czarnik-Matusewicz B, Pilorz S, Hawranek JP (2005) Temperature-dependent water structural transitions examined by near-IR and mid-IR spectra analyzed by multivariate curve resolution and two-dimensional correlation spectroscopy. Anal Chim Acta 544:15–25. https://doi.org/10.1016/j.aca.2005.04.040
da Sartori TAIC, Ferreira JA, Osiro D et al (2018) Formation of different calcium phosphate phases on the surface of porous Al2O3-ZrO2nanocomposites. J Eur Ceram Soc 38:743–751. https://doi.org/10.1016/j.jeurceramsoc.2017.09.014
Dehestani M, Ilver L, Adolfsson E (2012) Enhancing the bioactivity of zirconia and zirconia composites by surface modification. J Biomed Mater Res Part B Appl Biomater 100B:832–840. https://doi.org/10.1002/jbm.b.32647
Dele-Afolabi TT, Hanim MAA, Norkhairunnisa M et al (2017) Investigating the effect of porosity level and pore former type on the mechanical and corrosion resistance properties of agro-waste shaped porous alumina ceramics. Ceram Int 43:8743–8754. https://doi.org/10.1016/j.ceramint.2017.03.210
Dele-Afolabi T, Azmah Hanim MA, Mazlan N et al (2018a) Effect of agro-waste pore formers on the microstructure, hardness, and tensile properties of porous alumina ceramics. Int J Appl Ceram Technol 15:1060–1071. https://doi.org/10.1111/ijac.12874
Dele-Afolabi TT, Azmah Hanim MA, Norkhairunnisa M et al (2018b) Significant effect of rice husk and sugarcane bagasse pore formers on the microstructure and mechanical properties of porous Al2O3/Ni composites. J Alloys Compd 743:323–331. https://doi.org/10.1016/j.jallcom.2018.01.230
Dele-Afolabi TT, Azmah Hanim MA, Ojo-Kupoluyi OJ et al (2021) Tailored pore structures and mechanical properties of porous alumina ceramics prepared with corn cob pore-forming agent. Int J Appl Ceram Technol 18:244–252. https://doi.org/10.1111/ijac.13621
Dorozhkin SV (2010) Bioceramics of calcium orthophosphates. Biomaterials 31:1465–1485. https://doi.org/10.1016/j.biomaterials.2009.11.050
Dorozhkin SV (2012) Calcium orthophosphate coatings, films and layers. Prog Biomater 1:1. https://doi.org/10.1186/2194-0517-1-1
Dorozhkin SV (2016) Calcium orthophosphates (CaPO4): occurrence and properties. Prog Biomater 5:9–70. https://doi.org/10.1007/s40204-015-0045-z
Ebrahimi M, Botelho MG, Dorozhkin SV (2017) Biphasic calcium phosphates bioceramics (HA/TCP): Concept, physicochemical properties and the impact of standardization of study protocols in biomaterials research. Mater Sci Eng C 71:1293–1312. https://doi.org/10.1016/j.msec.2016.11.039
Faga MG, Vallée A, Bellosi A et al (2012) Chemical treatment on alumina—zirconia composites inducing apatite formation with maintained mechanical properties. J Eur Ceram Soc 32:2113–2120. https://doi.org/10.1016/j.jeurceramsoc.2011.12.020
Gadaleta SJ, Paschalis EP, Betts F et al (1996) Fourier transform infrared spectroscopy of the solution-mediated conversion of amorphous calcium phosphate to hydroxyapatite: new correlations between X-ray diffraction and infrared data. Calcif Tissue Int 58:9–16. https://doi.org/10.1007/s002239900004
Habraken W, Habibovic P, Epple M, Bohner M (2016) Calcium phosphates in biomedical applications: Materials for the future? Mater Today 19:69–87. https://doi.org/10.1016/j.mattod.2015.10.008
Hesaraki S (2016) Feasibility of alumina and alumina-silica nanoparticles to fabricate strengthened betatricalcium phosphate scaffold with improved biological responses. Ceram Int 42:7593–7604. https://doi.org/10.1016/j.ceramint.2016.01.167
Joint Committee on Powder Diffraction Standards J Joint Committee on Powder Diffraction Standards, JCPDS. International Center for Diffraction Data, and American Society for Testing and Materials, Powder Diffraction File (2017) Cards Numbers: 09-432, 73-1731, 09-348, 09-169, 25-1137 e 79-0423
Karageorgiou V, Kaplan D (2005) Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26:5474–5491. https://doi.org/10.1016/j.biomaterials.2005.02.002
Karampour H, Parsa MA, Moghadam AH et al (2022) Facile solution-based synthesis of impurity-free hydroxyapatite nanocrystals at ambient conditions. J Mater Res Technol 16:656–674. https://doi.org/10.1016/j.jmrt.2021.12.028
Karczewski K, Stepniowski W, Salerno M (2017) Amino acids aided sintering for the formation of highly porous FeAl intermetallic alloys. Materials (basel) 10:746. https://doi.org/10.3390/ma10070746
Kokubo T (1998) Apatite formation on surfaces of ceramics, metals and polymers in body environment. Acta Mater 46:2519–2527. https://doi.org/10.1016/S1359-6454(98)80036-0
Kolmas J, Marek D, Kolodziejski W (2015) Near-infrared (NIR) spectroscopy of synthetic hydroxyapatites and human dental tissues. Appl Spectrosc 69:902–912. https://doi.org/10.1366/14-07720
Kolos E, Ruys AJ (2015) Biomimetic coating on porous alumina for tissue engineering: characterisation by cell culture and confocal microscopy. Materials (basel) 8:3584–3606. https://doi.org/10.3390/ma8063584
Nunes FC, Maia MA, Santos KH et al (2021) Influence of Sr2+ in calcium phosphates formation on the surface of Al2O3/ZrO2nanocomposites. Ceram Int 47:30685–30690. https://doi.org/10.1016/j.ceramint.2021.07.247
Petit C, Meille S, Maire E et al (2016) Mechanical behaviour of a β-TCP ceramic with a random porosity: Study of the fracture path with X-ray tomography. J Eur Ceram Soc 36:3225–3233. https://doi.org/10.1016/j.jeurceramsoc.2016.05.001
Rambo CR, Muller FA, Muller L et al (2006) Biomimetic apatite coating on biomorphous alumina scaffolds. Mater Sci Eng C 26:92–99. https://doi.org/10.1016/j.msec.2005.06.003
Reddy GU, Reddy TR, Padmalatha KS (2014) Synthesis XRD TEM and optical absorption spectroscopic characterization of copper doped nano apatite. Prog NanotechnolNanomater 3:19–25
Røhl L, Larsen E, Linde F et al (1991) Tensile and compressive properties of cancellous bone. J Biomech 24:1143–1149. https://doi.org/10.1016/0021-9290(91)90006-9
Sabree I, Gough JE, Derby B (2015) Mechanical properties of porous ceramic scaffolds: influence of internal dimensions. Ceram Int 41:8425–8432. https://doi.org/10.1016/j.ceramint.2015.03.044
Salerno M, Loria P, Matarazzo G et al (2016) Surface morphology and tooth adhesion of a novel nanostructured dental restorative composite. Materials (basel) 9:1–8. https://doi.org/10.3390/ma9030203
Salomão R, Cardoso PH, Brandi J (2014) Gelcasting porous alumina beads of tailored shape and porosity. Ceram Int 40:16595–16601. https://doi.org/10.1016/j.ceramint.2014.08.017
Santos KH, Ferreira JA, Osiro D et al (2017) Influence of different chemical treatments on the surface of Al2O3/ZrO2nanocomposites during biomimetic coating. Ceram Int 43:4272–4279. https://doi.org/10.1016/j.ceramint.2016.12.069
Santos KH, Ferreira JA, Osiro D et al (2018) Plasma surface treatments of Al2O3/ZrO2nanocomposites and their influence on the formation and adhesion of calcium phosphates. Appl Surf Sci 456:552–560. https://doi.org/10.1016/j.apsusc.2018.06.188
Santos KH, Ferreira JA, Osiro D et al (2020) Influence of the cold plasma treatment on the Al2O3/ZrO2 nanocomposites surfaces. Appl Surf Sci 531:147206. https://doi.org/10.1016/j.apsusc.2020.147206
Sarkar D, Mohapatra D, Ray S et al (2007) Synthesis and characterization of sol-gel derived ZrO2 doped Al2O3nanopowder. Ceram Int 33:1275–1282. https://doi.org/10.1016/j.ceramint.2006.05.002
Schröter L, Kaiser F, Stein S et al (2020) Biological and mechanical performance and degradation characteristics of calcium phosphate cements in large animals and humans. Acta Biomater 117:1–20. https://doi.org/10.1016/j.actbio.2020.09.031
Seyedlar RM, Rezvani M, Barari S et al (2019) Synthesis of plate-like β-tricalcium phosphate nanoparticles and their efficiency in remineralization of incipient enamel caries. Prog Biomater 8:261–276. https://doi.org/10.1007/s40204-019-00126-y
Shavandi A, Bekhit AEA, Ali MA et al (2015) Development and characterization of hydroxyapatite/β -TCP/chitosan composites for tissue engineering applications. Mater Sci Eng C 56:481–493. https://doi.org/10.1016/j.msec.2015.07.004
Silva ADR, Rigoli WR, Osiro D et al (2018) Surface modification using the biomimetic method in alumina-zirconia porous ceramics obtained by the replica method. J Biomed Mater Res Part B Appl Biomater 106:2615–2624. https://doi.org/10.1002/jbm.b.34078
Silva ADR, Rigoli WR, Mello DCR et al (2019) Porous alumina scaffolds chemically modified by calcium phosphate minerals and their application in bone grafts. Int J Appl Ceram Technol 16:562–573. https://doi.org/10.1111/ijac.13153
Sureshbabu S, Komath M, Varma HK (2012) In situ formation of hydroxyapatite - alpha tricalcium phosphate biphasic ceramics with higher strength and bioactivity. J Am Ceram Soc 95:915–924. https://doi.org/10.1111/j.1551-2916.2011.04987.x
Tang X, Mao L, Liu J et al (2016) Fabrication, characterization and cellular biocompatibility of porous biphasic calcium phosphate bioceramic scaffolds with different pore sizes. Ceram Int 42:15311–15318. https://doi.org/10.1016/j.ceramint.2016.06.172
Thavornyutikarn B, Chantarapanich N, Sitthiseripratip K et al (2014) Bone tissue engineering scaffolding: computer-aided scaffolding techniques. Prog Biomater 3:61–102. https://doi.org/10.1007/s40204-014-0026-7
Toccafondi C, Dante S, Reverberi AP, Salerno M (2015) Biomedical applications of anodic porous alumina. Curr Nanosci 11:572–580. https://doi.org/10.2174/1573413711666150415225541
Uchida M, Kim H, Kokubo T et al (2002) Apatite-forming ability of a zirconia/alumina nano-composite induced by chemical treatment. J Biomed Mater Res Part A 60:277–282. https://doi.org/10.1002/jbm.10071
Wagoner Johnson AJ, Herschler BA (2011) A review of the mechanical behavior of CaP and CaP/polymer composites for applications in bone replacement and repair. Acta Biomater 7:16–30. https://doi.org/10.1016/j.actbio.2010.07.012
Wang Y (2016) Bioadaptability: an innovative concept for biomaterials. J Mater Sci Technol 32:801–809. https://doi.org/10.1016/j.jmst.2016.08.002
Workman J Jr, Weyer L (2012) Pratical guide and spectral atlas for interpretive near-infrared spectroscopy, 2nd edn. CRC, New York
**e R, Zhang D, Zhang X et al (2012) Gelcasting of alumina ceramics with improved green strength. Ceram Int 38:6923–6926. https://doi.org/10.1016/j.ceramint.2012.05.027
Xu W, Tian J, Liu Z et al (2019) Novel porous Ti35Zr28Nb scaffolds fabricated by powder metallurgy with excellent osteointegration ability for bone-tissue engineering applications. Mater Sci Eng C 105:110015. https://doi.org/10.1016/j.msec.2019.110015
Yoo D (2013) New paradigms in hierarchical porous scaffold design for tissue engineering. Mater Sci Eng C 33:1759–1772. https://doi.org/10.1016/j.msec.2012.12.092
Zhang J, Liu W, Schnitzler V et al (2014) Calcium phosphate cements for bone substitution: Chemistry, handling and mechanical properties. Acta Biomater 10:1035–1049. https://doi.org/10.1016/j.actbio.2013.11.001
Zhang Y, Shao H, Lin T et al (2019) Effect of Ca/P ratios on porous calcium phosphate salt bioceramic scaffolds for bone engineering by 3D gel-printing method. Ceram Int 45:20493–20500. https://doi.org/10.1016/j.ceramint.2019.07.028
Zyman Z, Goncharenko A, Rokhmistrov D (2017) Kinetics and mechanisms of the transformation of precipitated amorphous calcium phosphate with a Ca/P ratio of 1:1 to calcium pyrophosphates. J Cryst Growth 478:117–122. https://doi.org/10.1016/j.jcrysgro.2017.08.031
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This study was financed by the National Council for Scientific and Technological Development (CNPq) [151281/2015-7; 102905/2015-0].
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Lavagnini, I.R., Campos, J.V., Osiro, D. et al. Influence of alumina substrates open porosity on calcium phosphates formation produced by the biomimetic method. Prog Biomater 11, 263–271 (2022). https://doi.org/10.1007/s40204-022-00193-8
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DOI: https://doi.org/10.1007/s40204-022-00193-8